Collagen Gel을 이용한 사람의 고환 조직배양에 관한연구

A Study on Growth of Human Testicular Tissue in 3-Dimensional Collagen Gel Tissue Culture

  • 이충현 (경희대학교 의과대학 비뇨기과학교실) ;
  • 이상철 (경희대학교 의과대학 비뇨기과학교실) ;
  • 이선주 (경희대학교 의과대학 비뇨기과학교실) ;
  • 손준웅 (경희대학교 의과대학 비뇨기과학교실) ;
  • 장성구 (경희대학교 의과대학 비뇨기과학교실) ;
  • 김진일 (경희대학교 의과대학 비뇨기과학교실) ;
  • 채수응 (경희대학교 의과대학 비뇨기과학교실)
  • Lee, Choong-Hyun (Department of Urology, School of Medicine, Kyung Hee University) ;
  • Lee, Sang-Cheol (Department of Urology, School of Medicine, Kyung Hee University) ;
  • Lee, Sun-Joo (Department of Urology, School of Medicine, Kyung Hee University) ;
  • Sohn, Joon-Woong (Department of Urology, School of Medicine, Kyung Hee University) ;
  • Chang, Sung-Goo (Department of Urology, School of Medicine, Kyung Hee University) ;
  • Kim, Jin-Il (Department of Urology, School of Medicine, Kyung Hee University) ;
  • Chai, Soo-Eung (Department of Urology, School of Medicine, Kyung Hee University)
  • 발행 : 1993.06.04

초록

A recently developed collagen gel culture technique has been applied to study on growth in tissue of human testicular tissue. Minimum Eagle's medium supplemented with amino acid, 10% Fetal Bovine Serum and 0.1mM non-essential amino acid are emploid. Tissue fragments on collagan gel are fixed at time intervals for the histologic findings of testis. The mature spermatids are maintained for 2 weeks and can be observed until four weeks. But the rate of glucose consumption is increased contrary to histologic findings.

키워드