Clinical and Experimental Reproductive Medicine
- Volume 20 Issue 1
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- Pages.53-56
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- 1993
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
A Study on Growth of Human Testicular Tissue in 3-Dimensional Collagen Gel Tissue Culture
Collagen Gel을 이용한 사람의 고환 조직배양에 관한연구
- Lee, Choong-Hyun (Department of Urology, School of Medicine, Kyung Hee University) ;
- Lee, Sang-Cheol (Department of Urology, School of Medicine, Kyung Hee University) ;
- Lee, Sun-Joo (Department of Urology, School of Medicine, Kyung Hee University) ;
- Sohn, Joon-Woong (Department of Urology, School of Medicine, Kyung Hee University) ;
- Chang, Sung-Goo (Department of Urology, School of Medicine, Kyung Hee University) ;
- Kim, Jin-Il (Department of Urology, School of Medicine, Kyung Hee University) ;
- Chai, Soo-Eung (Department of Urology, School of Medicine, Kyung Hee University)
- 이충현 (경희대학교 의과대학 비뇨기과학교실) ;
- 이상철 (경희대학교 의과대학 비뇨기과학교실) ;
- 이선주 (경희대학교 의과대학 비뇨기과학교실) ;
- 손준웅 (경희대학교 의과대학 비뇨기과학교실) ;
- 장성구 (경희대학교 의과대학 비뇨기과학교실) ;
- 김진일 (경희대학교 의과대학 비뇨기과학교실) ;
- 채수응 (경희대학교 의과대학 비뇨기과학교실)
- Published : 1993.06.04
Abstract
A recently developed collagen gel culture technique has been applied to study on growth in tissue of human testicular tissue. Minimum Eagle's medium supplemented with amino acid, 10% Fetal Bovine Serum and 0.1mM non-essential amino acid are emploid. Tissue fragments on collagan gel are fixed at time intervals for the histologic findings of testis. The mature spermatids are maintained for 2 weeks and can be observed until four weeks. But the rate of glucose consumption is increased contrary to histologic findings.