Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Studies on Quality Control by Frozen-Thaw 2-Cell Mouse Embryos

냉동보존된 생쥐배아를 이용한 정도관리에 관한 연구

  • Han, Sun-Nam (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Kim, Hyang-Mee (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Jung, Hae-Won (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Oh, Seung-Eun (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Son, Young-Soo (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Yu, Han-Ki (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Ahn, Jung-Ja (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University) ;
  • Woo, Bock-Hee (Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University)
  • 한선남 (이화여자대학교 의과대학 산부인과학교실) ;
  • 김향미 (이화여자대학교 의과대학 산부인과학교실) ;
  • 정혜원 (이화여자대학교 의과대학 산부인과학교실) ;
  • 오승은 (이화여자대학교 의과대학 산부인과학교실) ;
  • 손영수 (이화여자대학교 의과대학 산부인과학교실) ;
  • 유한기 (이화여자대학교 의과대학 산부인과학교실) ;
  • 안정자 (이화여자대학교 의과대학 산부인과학교실) ;
  • 우복희 (이화여자대학교 의과대학 산부인과학교실)
  • Published : 1993.08.31
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Abstract

These studies were carried out to investigate the optimal freezing protocol for 2 cell mouse embryos and to find the probability of quality control with 2-cell embryos frozen. The embryos showed the best survival by the protocol composed of a freezing solution with the cryoprotectants(1.5M propanediol + 0.1M sucrose), and a 2-steop thawing method(room temperature, 20 sec-37$^{\circ}C$, 20 sec). The developmental ability of frozen-thaw 2-cell embryos did not differ from that of fresh 2-cell embryos in m-KRB medium with 0.4% bovine serum albumin. But development of frozen-thaw embryos was depended on the supplements of the medium. In the albumin-free medium, the developmental rate(rate of blastocysts) was significantly reduced, compared with that in the medium with 0.4% BSA. Also, when frozen-thaw embryos were cultured in the meduim with human fetal cord serum(HCS), the developmental rate of frozen-thaw embryos was sligtly reduced, compared with that of fresh 2-cell embryos. Finally, frozen-thaw 2-cell mouse embryos were more sensitive to the toxic agent of disposable-plastic syringe. Therefore, toxicity of medium could be effectively detected by frozen-thaw 2-cell mouse embryos.

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