Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Comparison among the Sperm Preparation Methods on the Human Spermatozoa

인간정자에 있어서 정자처리법의 비교

  • Pang, Myung-Geol (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Chung, Ku-Min (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Shin, Chang-Jae (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Kim, Jung-Gu (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Moon, Shin-Yong (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Chang, Yoon-Seok (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Lee, Jin-Yong (Department of Obstetrics and Gyecology. College of Medicine. Seoul National University) ;
  • Lee, Sang-Hoon (Department of Obstetrics and Gynecology. College of Medicine. Chung-Ang University) ;
  • Chung, Yung-Chai (Department of Animal Science, Chung-Ang University) ;
  • Kim, Chang-Keun (Department of Animal Science, Chung-Ang University)
  • 방명걸 (서울대학교 의과대학 산부인과학교실) ;
  • 정구민 (서울대학교 의과대학 산부인과학교실) ;
  • 신창재 (서울대학교 의과대학 산부인과학교실) ;
  • 김정구 (서울대학교 의과대학 산부인과학교실) ;
  • 문신용 (서울대학교 의과대학 산부인과학교실) ;
  • 장윤석 (서울대학교 의과대학 산부인과학교실) ;
  • 이진용 (서울대학교 의과대학 산부인과학교실) ;
  • 이상훈 (중앙대학교 의과대학 산부인과학교실) ;
  • 정영채 (중앙대학교 산업대학 축산학과) ;
  • 김창근 (중앙대학교 산업대학 축산학과)
  • Published : 1993.08.31
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Abstract

Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.

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