A STUDY ON THE CYTOTOXICITY OF ROOT CANAL ANTISEPTIC SOLUTIONS

근관소독제의 세포독성에 관한 연구

  • Kim, Jae-Gu (Department of Conservatice Dentistry, College of Dentistry, Wonkwang University) ;
  • Im, Mi-Kyung (Department of Conservatice Dentistry, College of Dentistry, Wonkwang University)
  • 김재구 (원광대학교 치과대학 치과보존학교실) ;
  • 임미경 (원광대학교 치과대학 치과보존학교실)
  • Published : 1993.04.25

Abstract

Two functions of root canal medicaments and irrigants are to reduce microorganisms and to encourge the repair of apical tissues. The biocompatibility of endodontic materials has been tested using in vitro cell culture techniques. The purpose of this study Was to evaluate and compare the cytotoxic effects of 2 root canal irrigation solutions and 4 antiseptics on HEp-2 and McCoy cells. Two irrigation solutions were sodium hypochlorite. $H_2O_2$ and 4 antiseptics were povidone, ethanol, glutaraldehyde and benzalkonium chloride. Each solutions were serially diluted to 1:1, 1:10, 1:$10^2$, 1:$10^3$, 1:$10^4$, 1:$10^5$, 1:$10^6$. And each diluted solutions were added to the cells and cytotoxic effects were measured with the absorbance of formazan formed cells by ELISA READER. The results were as follows : 1. Benzalkonium chloride was the most cytotoxic on HEp-2 cell. (P<0.05) 2. $H_2O_2$ was the most cytotoxic on McCoy cell. (P<.05) 3. Povidone and ethanol showed mild cytotoxic effect on HEp-2 and McCoy cell. (P<0.05).

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