Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus

축우 부루셀라병의 ELISA 진단법에 관한 연구

  • Lim, Yoon-kyu (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Doo-sick (Department of Veterinary Medicine, Cheju National University) ;
  • Park, Jun-hong (Department of Veterinary Medicine, Cheju National University) ;
  • Yang, Ki-chun (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Seung-ho (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Kong-sick (Cheju Veterinary Service Laboratory) ;
  • Hyun, Kwan-jong (Cheju Veterinary Service Laboratory) ;
  • Kim, Woo-tack (Cheju Veterinary Service Laboratory) ;
  • Lee, Yong-soon (CoIlege of Veterinary Medicine, Seoul National University)
  • Received : 1992.09.25
  • Published : 1993.01.30
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Abstract

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were $0.4{\mu}g/100{\mu}{\ell}$ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases(10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle.

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