n-3 Highly Unsaturated Fatty Acid Requirement of the Korean Rockfish Sebastes schlegeli

조피볼락 Sebastes schlegeil의 n-3계 고도불포화지방산 요구량

  • LEE Sang-Min (National Fisheries Research and Development Agency) ;
  • LEE Jong Yun (National Fisheries Research and Development Agency) ;
  • KANG Yong Jin (National Fisheries Research and Development Agency) ;
  • YOON Ho-Dong (National Fisheries Research and Development Agency) ;
  • HUR Sung Bum (Department of Aquaculture, National Fisheries University of Pusan)
  • Published : 1993.09.01

Abstract

In order to investigate the n-3 highly unsaturated fatty acids (n-3HUFA) requirement of the Korean rockfish Sebastes schlegeli, two experiments were conducted in the flush-out aquarium system. 1. Effects of different dietary fatty acids on growth and feed efficiency Efficacy of different fatty acids on the Korean rockfish was investigated by feeding diets containing each of the different fatty acids, 12:0, 18:1n-9, 18:2n-6, 18:3n-3, and n-3HUFA for 9 weeks. The best growth and feed efficiency were obtained from the fish fed diet containing n-3HUFA (P<0.05). 2. n-3HUFA requirement Requirement of dietary n-3HUFA for the Korean rockfish (5.9 g in mean body weight) was investigated with the test diets containing different levels of n-3HUFA ranging from $0\%$ to $4.0\%$ at $8\%$ dietary lipid level. After 6 weeks of feeding experiment, fish performance and fatty acid composition of liver were studied. Growth was significantly improved with increasing dietary n-3HUFA level up to the $0.9\%$ in the diet (P<0.05). Higher values of lipid content, 18:1/n-3HUFA ratio of polar lipid of liver and hepatosomatic index were observed in the fish fed n-3HUFA deficient diets. The groups of fish fed lower levels of dietary n-3HUFA showed higher 18:1 and love. n-3HUFA (EPA+DHA) levels in polar lipid of the liver. The data obtained in these experiments indicated that dietary n-3HUFA was essential for the Korean rockfish, and required level of n-3HUFA was around $0.9\%$ in diet.

1. 조피볼락의 적정성장에 필요한 지방산을 조사하기 위해 사료의 지방산종류 (fat-free, 12:0, 18:1n-9, 18:2n-6, 18:3n-3 및 n-3HUFA)가 다른 6종의 실험사료로 44g 전후의 조피볼락을 9주간 사육한 결과, fat-free, 12:0, 18:1n-9, 18:2n-6 및 18:3n-3 첨가구보다 n-3HUFA 첨가구가 성장 및 사료효율에서 좋은 결과를 보였다. 2. 조피볼락의 n-3HUFA 요구량을 구명하기 위해, 사료지질 $8\%$ 범위에서 기본지질을 우지로 하여 정제 지방산 (n-3HUFA $85\%$)을 첨가, n-3HUFA 함량이 $0{\sim}4.0\%$가 되도록 조정한 10종의 실험사료로 평균 체중 5.9g의 조피볼락 치어를 6주간 사육한 후, 성장 및 체성분의 변화를 조사하였다. 증중률 ($117{\sim}297\%$) 및 사료효율($67{\sim}96\%$)모두 n-3HUFA 무첨가 사료에서 가장 낮은 값을 나타내었으며, 사료 n-3HUFA 함량의 증가와 더불어 그 효과가 현저히 개선되다가 $0.9\%$ 이후에는 더이상 개선되지 않았다. 따라서, 조피볼락의 n-3HUFA 요구량은 $0.9\%$로 추정되었다. 사료의 n-3HUFA 함량이 증가함에 따라 간지질 함량과 간중량 비는 감소하는 경향을 보였으며, 간 glycogen 함량은 증가하였다. 간 극성지질의 지방산조성은 사료의 지방산조성에 따라 변화하여, 사료의 n-3HUFA가 증가함에 따라 18:1은 감소하고, EPA와 DHA같은 n-3HUFA는 증가하였다. 그리고 간 극성지질의 18:1/n-3HUFA 비는 사료의 n-3HUFA 증가와 더불어 감소하였다.

Keywords