EFFECTS OF CYTOKINES ON THE CELL PROLIFERATION OF CYTOLYTIC T CELL LINE CTLL - 2

Cytolytic T cell line CTLL - 2의 세포증식에 미치는 cytokine의 효과

  • Seo, Yang-Ja (Dept. of Periodontology, College of Dentistry, Chonbuk National University) ;
  • Lee, In-Kyu (Dept. of Periodontology, College of Dentistry, Chonbuk National University) ;
  • Lee, Jin-Young (Dept. of Periodontology, College of Dentistry, Chonbuk National University) ;
  • Oh, Kwi-Ok (Dept. of Phamacology, College of Dentistry, Chonbuk National University) ;
  • Kim, Hyung-Seop (Dept. of Periodontology, College of Dentistry, Chonbuk National University)
  • 서양자 (전북대학교 치과대학 치주과학교실) ;
  • 이인규 (전북대학교 치과대학 치주과학교실) ;
  • 이진용 (전북대학교 치과대학 치주과학교실) ;
  • 오귀옥 (전북대학교 치과대학 약리학교실) ;
  • 김형섭 (전북대학교 치과대학 치주과학교실)
  • Published : 1993.11.30

Abstract

Abnormalities of the T cell subsets have been detected in the immunologically mediated disease sites such as periodontal lesions which are attributable to the regulatory effect of cell differentiation and specific chemokinetic effect of various cytokines. Macrophage Inflammatory protein$(MIP)-1{\alpha}$ and gammain terferon$({\gamma}-IFN)$ serve as important immunoregulatory molecules through which growth and differentiation of specific T cell subsets are known to be negatively regulated. Murine cytolytic T cell line CTLL-2 were used to perform the [$^3H$]-thymidine incorporation test, by which we obtained more comprehensive view in regulatory actions of cytokines on the T cell subset proliferation. 1. $rMIP-{\alpha}$(200ng/ml) and $r{\gamma}-IFN$(100U/ml) appreared to suppress the proliferation rate to CTLL-2 by 74 and 86% respectively, and the suppressive action of two cytokines were synergisic. 2. Culture supernatant of anti-CD3 mAb-stimulated mouse splenocyte enhanced the proliferation rate of CTLL-2 up to 10-fold with dose-dependent manner. However, culture supernatant of unstimulated splenocyte showed only 2-fold increase in the proliferation rate. 3. CTLL-2 cell proliferation was strictly IL-2 dependent.

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