Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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STUDIES ON THE MACROPHAGE INFLAMMATORY $PROTEIN-1{\alpha}$ IN BONE MARROW, SPLEEN, AND MACROPHAGE

비장, 골수세포 및 대식세포에서의 Macrophage Inflammatory $Protein-1{\alpha}(MIP-1{\alpha})$ 에 관한 연구

  • Song, In-Taeck (Dept. of Periodontology, College of Dentistry, Chonbuk National University) ;
  • Oh, Kwi-Ok (Dept. of Phamacology, College of Dentistry, Chonbuk National University) ;
  • Kim, Hyung-Sup (Dept. of Periodontology, College of Dentistry, Chonbuk National University)
  • 송인택 (전북대학교 치과대학 치주과학교실) ;
  • 오귀옥 (전북대학교 치과대학 약리학교실) ;
  • 김형섭 (전북대학교 치과대학 치주과학교실)
  • Published : 1993.04.30
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Abstract

Macrophage inflammatory $protein-1{\alpha}(MIP-1{\alpha})$ from activated T cell or macrophage, which is small inducible cytokine of unkown biological function, has been shown to display inflammation chemokinetic activities, as well as myelosuppressive effect on more immature progenitor cells. In this paper we show the $MIP-1{\alpha}$ mRNA expression and the presence of $MIP-1{\alpha}$ binding sites from murine macrophage cell line RAW 264.7, and primary cells of mouse bone marrow and spleen. $MIP-1{\alpha}$ mRNA was induced from LPS-stimulated RAW 264.7, but not inhibited by cyclosporin A treatment, and also was expressed from mouse splenocyted and bone marrow cell which were not increased by ferritin or lactoferrin treatment. The results of receptor binding assay showed that radiolabeled RAW 264.7 cell with kd value of 0.91 nM, and binding sites per cell of 378. bone marrow cell and splenocyte also appeared to have $MIP-1{\alpha}$ binding sites 33 and 11 per cell, respectiviely.

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