Applied Microscopy
- Volume 23 Issue 2
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- Pages.11-26
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- 1993
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Fine Structure on the Pigment Epithelial Cell and the Bruch's Membrane of the Rat Retina after X-Irradiation
X-선 조사를 받은 흰쥐 망막의 색소상피세포와 기저복합층의 미세구조
- Ko, Jeong-Sik (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
- Shin, Ki-Ho (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
- Ahn, E.Tay (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
- Yang, Nam-Gil (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
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Park, Kyung-Ho
(Department of Anatomy, College of Medicine, Soonchunhyang University) ;
- Kim, Jin-Gook (Department of Anatomy, College of Medicine, Soonchunhyang University)
- 고정식 (순천향대학교 의과대학 해부학교실) ;
- 신기호 (순천향대학교 의과대학 해부학교실) ;
- 안의태 (순천향대학교 의과대학 해부학교실) ;
- 양남길 (순천향대학교 의과대학 해부학교실) ;
-
박경호
(순천향대학교 의과대학 해부학교실) ;
- 김진국 (순천향대학교 의과대학 해부학교실)
- Published : 1993.09.01
Abstract
This experiment was performed to study the morphological responses of the pigment epithelial cell and the Bruch's membrane of the retina of rat following X-ray irradiation. Male rats were divided into normal and experimental groups. The heads of the rats, under sodium thiopental anesthesia, were exposed to 3,000 rads or 6,000 rads of radiation in a single dose, respectively. The source was a Mitsubishi Linear Accelerator ML-4MV. The target to skin distance was 80cm, and the. dose rate was 200 rads/min. The experimental groups were sacrificed on the 6th hour, 2nd and 6th day after X-ray irradiation. Under anesthesia, 1% glutaraldehyde-1% paraformaldehyde solution(0.1M Millonig's phosphate buffer, pH 7.3) was perfused through the left ventricle and ascending aorta. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde(0.1M Millonig's phosphate buffer, pH 7.3) and 1% osmium tetroxide(0.1M Millonig's phosphate buffer, pH7.3), and embedded in araldite mixture. The ultrathin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100 CX-II electron microscope. The results were as follow; 1. The morphological changes of the pigment epithelial cells were not pronounced after exposure to 3,000 rads of X-ray. But on the 6th hour after exposure to 6,000 rads of X-ray, bulging nuclear membrane protruding into the cytoplasm and nuclear chromatin clumped into numerous masses along the nuclear membrane were observed. At the 2nd and 6th day post-irradiation, partial cytolysis or necrosis were seen. 2. The thickness of the Bruch's membrane of the experimental groups were increased in the time and dose range covered by this study, and splitting or diffusing basal laminae of the choriocapillary layer were observed frequently in the experimental group. Above results suggest that large amount(6,000 rads) of head irradiation induce direct hazardous effects on the pigment epitherial cells and Bruch's membrane of the retina of the rat, but pigment epithelial cells are more radioresistant than Bruch's membrane.
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