한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제21권4호
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- Pages.381-391
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- 1993
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
세포조직배양법을 이용한 쥐 인공피부의 개발
Development of Dermal Equivalent Using Mouse Fibroblasts
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Yang, Eun-Kyoung
(Dept. of Chemical Engineeering, Dongguk University) ;
- Lee, Jae-Ho (Dept. of Microbial Engineering, Konkuk University) ;
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Choe, Tae-Boo
(Dept. of Microbial Engineering, Konkuk University) ;
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Park, Jung-Keug
(Dept. of Chemical Engineeering, Dongguk University)
- 발행 : 1993.08.01
초록
As the first stage of development of an artificial skin, fibroblasts were cultured in the collagen matrices to make a living dermal equivalent. Mouse embryonic fibroblasts were incorporated into a collagen matrices on plastic dishes containing concentrated DMEM culture media supplemented with sodium bicarbonate, hepes, antibiotics and fetal bovine serum. As the growth stimulation components, glycosaminoglycans were added: hyaluronic acid, chondroitin sulfate, heparin, chitosan were incorporated into the media at a concentration of either 1% or 5% w/w/ to collagen in order to investigate the effect on development of dermal equivalent. After the few days of incubation, gel matrics were contracted and firm dermal equivalent were formed. And the keratinocytes were cultured on top of dermal equivalent and make a three dimensional artificial skin tissue.