한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제21권4호
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- Pages.354-365
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- 1993
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
유가식 생물반응기에서의 용존산소농도 및 비성장속도의 제어
Control of dissolved Oxygen Concentration and Specific Growth Rate in Fed-batch Fermentation
- 김창겸 (한국과학기술원 생물공정연구센터 및 화학공학과) ;
- 이태호 (한국과학기술원 생물공정연구센터 및 화학공학과) ;
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이승철
(한국과학기술원 생물공정연구센터 및 화학공학과) ;
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장용근
(한국과학기술원 생물공정연구센터 및 화학공학과) ;
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장호남
(한국과학기술원 생물공정연구센터 및 화학공학과)
- Kim, Chang-Gyeom (BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology) ;
- Lee, Tae-Ho (BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology) ;
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Lee, Seung-Cheol
(BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology) ;
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Chang, Yong-Keun
(BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology) ;
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Chang, Ho-Nam
(BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology)
- 발행 : 1993.08.01
초록
A novel control method with automatic tuning of PID controller parameters has been developed for efficient regulation of dissolved oxygen concentration in fed-batch fermentations of Escherichia coli. Agitation speed and oxygen partial pressure in the inlet gas stream were chosen to be the manipulated variables. A heuristic reasoning allowed improved tuning decisions from the supervision of control performance indices and it coule obviate the needs for process assumptions or disturbance patterns. The control input consisted of feedback and feedforword parts. The feedback part was determined by PID control and the feedforward part is determined from the feed rate. The proportional gain was updated on-line by a set of heuristics rules based on the supervision of three performance indices. These indices were output error covariance, the average value of output error, and input covariance, which were calculated on-line using a moving window. The integral and derivative time constants were determined from the period of output response. The specific growth rate was maintained at a low level to avoid acetic acid accumulation and thus to achieve a high cell density. The specific growthe rate was estimated from the carbon dioxide evolution rate. In fed-batch fermentation, the simutaneous control of dissolved oxygen concentration (at 0.2; fraction of saturated value) and specific growth rate (at 0.25