한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제21권4호
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- Pages.336-341
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- 1993
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
금속 친화성 액 이상분계 시스템에서 단백질의 분배 향상
Enhanced Partitioning of Proteins in Metal-Affinity Aqueous Two-Phase Systems
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Chung, Bong-Hyun
(Biochemical Process Lab., Genetic Engineering Research Institute, KIST) ;
- Park, Young-Hoon (Biochemical Process Lab., Genetic Engineering Research Institute, KIST)
- 발행 : 1993.08.01
초록
In metal-affinify aqueous two-phase systems, protein partitioning is affected by a variety of parameters such as pH, the number of surface-accessible histidines, and the amount and partition coefficient of metallated polythylene glyco(PEG) ligand. To enhance partitioning of proteins with surface-accessible histidines, we have synthesized and used a (Cu(II)-ininodiacetic acid)