Callus Induction and Plant Regeneration from Leaf Tissue. Culture of $\emph{Aralia elata}$ S.

두릅의 엽조직배양에 의한 Callus유기 및 식물체 재분화

This study was conducted to determine the optimum culture conditions for inducing callus and regenerating plantlets from cultured leaf tissues of Aralia elata. Young leaf tissues(1cm) of A. elata plant were cultured on MS medium supplemented with 2, 4-D and Thidiazuron. Embryogenic callus was induced along the leaf veins, more efficiently on the medium containing 1.0mg /1 Thidiazuron in 4 weeks after culture initiation. Calli were subcultured to proliferate on MS media containing 2, 4-D, Dicamba, Picloram, and Thidiazuron. Callus was better proliferated on the medium containing Dicamba than on the others.. However, callus subcultured on the medium containing Thidiazuron was more embryogenic and light green-colored, of which some showed embryoid-like structure on the surface. Hormone-free medium was more efficient to regenerate plantlets than media supplemented with Kinetin, BA, and Thidiazuron.

본 연구는 기내에서 두릅의 잎조직배양을 통한 효율적인 종묘대량증식체계를 확립하기 위한 기초연구로서 엽내으로부터 캘루스 유기 및 식물체 재분화를 시도한 결과이다. 1. 유연조직으로부터 캘루스 유기는 1.0mg/1 Thidiazuron 첨가배지에서 70%의 유기율을 보였다. 2. 0.1～0.2mg/1 Dicamba 및 1.0mg/1 Picloram 첨가가 캘루스의 증식에 효과가 있었다. 3. 호르몬 첨가배지에서는 식물체 재분화가 이루어지지 않은 반면 Hormon-free 배지에서 shoot 및 root의 분화가 이루어져 완전한 식물체를 얻었다.