Journal of Microbiology and Biotechnology
- Volume 3 Issue 4
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- Pages.224-231
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- 1993
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Sequence Analysis and Expression of Xylanase Gene (xynY) from Alkalophilic Bacillus sp. YC-335
- Park, Young-Seo (Department of Food Engineering, Yonsei University) ;
- Yum, Do-Young (Department of Food Engineering, Yonsei University) ;
- Kim, Jin-Man (Department of Food Engineering, Yonsei University) ;
- Bai, Dong-Hoon (Department of Food Engineering, Dankook University)
- Published : 1993.12.01
Abstract
The nucleotide sequence of the xylanase gene (xynY) from alkalophilic Bacillus sp. YC-335 was determined and analyzed. An open reading frame of 1, 062 base pairs for xynY gene was observed and encoded for a protein of 354 amino acids with a molecular weight of 38, 915. S1 nuclease mapping showed that the transcription initiation sites of the xynY gene were different in Bacillus sp. YC-335 and Escherichia coli HB101 (pYS55). S1 mapping also showed that -10 region of the xynY gene recognized by RNA polymerases of E. coli and Bacillus sp. YC-335 were TACAGT and TATGAT , respectively. A ribosome binding site sequence with the free energy of -17.0 Kcal/mol was observed 9 base pairs upstream from the unusual initiation codon, TTG. The proposed signal sequence consisted of 27 amino acids, 2 of which were basic amino acid residues and 21 were hydrophobic amino acid residues. When the amino acid sequences of xylanases were compared, Bacillus sp. YC-335 xylanase showed more than 50% homology with xylanases from B. pumilus, B. subtilis, and B. circulans.
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