Journal of Microbiology and Biotechnology
- Volume 3 Issue 3
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- Pages.188-193
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- 1993
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of Superoxide Dismutase from Pseudomonas polycolor
- LEE SANG-OK (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
- IL-CHUN SEO (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
- SOOK-HYUN CHUNG (Department of Food Engineering, Dongseo University) ;
- TAE-HO LEE (Department of Microbiology, College of Natural Sciences, Pusan National University)
- Published : 1993.09.01
Abstract
Superoxide dismutase (SOD) was purified from Pseudomonas polycolor to an electrophoretically homogeneous state and partially characterized. SOD was purified by ammonium sulfate fractionation, column chromatography on DEAE-Sephadex A-50, phenyl-Toyopearl 650 M, and gel filtration on Sephadex G-100. The molecular weight and subunit molecular weight of the purified enzyme were estimated to be 40, 000 and 20, 000, respectively. The purified enzyme remained stable at pH 9.0~11.0,