Journal of Microbiology and Biotechnology
- 제3권1호
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- Pages.31-38
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- 1993
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Enzymatic Characteristics of an Extracellular Agarase of Cytophaga sp. KY-1 and Molecular Cloning of the Agarase gene
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Kim, Young-Ho
(Department of Microbiology, College of Natural Science Kyungpook National University) ;
- Kim, Youn-Sook (Department of Microbiology, College of Natural Science Kyungpook National University) ;
- Lee, Jae-Ran (Department of Microbiology, College of Natural Science Kyungpook National University) ;
- Lee, Eun-Kyung (Department of Microbiology, College of Natural Science Kyungpook National University)
- 발행 : 1993.03.01
초록
A bacterial strain KY-l isolated from sewage was able to produce an extracellular agarase(agarose 4-glycanohydrolase. EC 3.2.1.81). The strain KY-1 was identified as Cytophaga fermentans subsp. agarovorans based on its morphological and physiological characteristics. The agarase was purified by ammonium sulfate precipitation followed by DEAE-Sephadex A-50. Bio-Gel P-100. and CM-Cellulose column chromatography. The molecular weight of the purified enzyme was 24 kDa by SDS-polyacrylamide gel electrophoresis. The optimum temperature and pH for the enzyme activity were 30^{circ}C and 7.5, respectively. The enzyme activity was significantly inhibited in the presence of 0.1 mM