Anticlastogenic Effects of Galangin against N-Methyl-N′-nitro-N-nitrosoguanidine-induced Micronuclei in Bone-marrow Cells of C57BL/6 Mice

  • Lee, Su-Jun (College of Natural Sciences, Kangweon National University) ;
  • Kwon, Chang-Ho (College of Pharmacy, Chungang University) ;
  • Kim, Kyeong-Ho (College of pharmacy, Kangweon National University) ;
  • Sohn, Dong-Hun (College of Pharmacy, Chungang University) ;
  • Heo, Moon-Young (College of pharmacy, Kangweon National University) ;
  • William w. au, William-W.-Au (Department of Preventive Medicine adn Community Health, University of Texas Medical Branch)
  • Published : 1993.12.01

Abstract

The anticlastogenic effect of galangin, flavonoid derivative, was studied in vivo micronucleus test using C57BL/6 mice. The frequencies of micronuclei induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in bone-marrow cells of C57BL/6 mice were significantly decreased by the simultaneous treatment or multiple pre-treatment of galangin. When galangin was orally administered at 0, 0.1, 1.0, or 10.0 mg/kg twice with 24 hr interval, together with intraperitoneally administered MNNG, there were suppressive effects in the tested doses. The most marked suppressive effect was observed in the treatment group of 1.0 mg/kg (64.5%), not in the treatment group of 10.0 mg/kg (36.3%). When galangin was multiply administered at 1/7 or 1 mg/kg for 7 days respectively, galangin showed higher suppressive effect in the treatment group of 1/7 mg/kg (23.5%) rather than in the treatment group of 1 mg/kg (13.5%). In another experiment, galangin was administered at 0.001, 0.01 or 0.1 mg/kg for 1 month respectively. The suppressive effects in one month treatment gradually increased with dose-dependent manner, although suppressive effects were not high. The results showed that galangin was effective in suppressing the frequencies of micronuclei induced by MNNG. Our study indicates that galangin is a potent anticlastogenic agent against MNNG.

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