Comparative Study on the HPLC Determination of Aflatoxins Coupled with Extraction and Clean-up Methods

Aflatoxin 분석법에 관한연구;추출 및 정제방법의 비교

  • 김종규 (계명대학교 자연과학대학 공중보건학과)
  • Published : 1993.12.01

Abstract

Extraction and Clean-up procedures coupled with quantitation by high performance liquid chromatography(HPLC) was evaluated for the detection of 4 aflatoxins, B1, B2, G1 and G2, in peanut butter. The Sep-pak clean-up method showed poorer separation and repeatability than did the modified DeVries' and an immunoaffinity column clean-up methods. No significant difference of detected aflatoxins between the affinity column clean-up and modified DeVries' method. The coefficients of variation for the 4 aflatoxins were ranging from 6.3∼32.3 by the modified DeVries' method and 5.3∼9.8 by the affinity column clean-up.

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