Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 8 Issue 2
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- Pages.151-157
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- 1993
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Study on Production of Cloned Animals by Recycling Nuclear Transplantation I. Activation of Nuclear Recipient Oocytes by Electrostimulation in Rabbits
반복핵이식에 의한 복제동물 생산에 관한 연구 I. 토끼 수핵난자의 전기자극에 의한 활성화
Abstract
The present study was undertaken to determine the optimal condition for parthenogenetic activation of rabbit oocytes by electric stimulation in vitro in an attempt to develop nuclear transplantation techniques for cloning mammalian embryos and animals. Freshly ovulated oocytes were collected from superovulated rabbits from 13 to 26 hrs. after hCG injection. The cumulus-free oocytes were activated parthenogetically by repeated stimuli of square direct electric pulses in O.3M mannitol solution. After applying electric stimulations of different voltages, pulse durations and pulse times, all of the oocytes were cultured in TCM-199 with 10% FCS for 96 hours in a 5%
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