Ashbya gossypii로부터 riboflavin 대량생산을 위한 배지 최적화와 유가식 배양

Media Optimization and Fed-Batch Fermentation for Riboflavin Overproduction by Ashbya gossypii

  • 남수완 (한국과학기술연구원 유전공학연구소) ;
  • 장형욱 (한국과학기술연구원 유전공학연구소) ;
  • 반재구 (한국과학기술연구원 유전공학연구소) ;
  • 민태익 (한국과학기술연구원 유전공학연구소) ;
  • 김익환 (한국과학기술연구원 유전공학연구소)
  • 발행 : 1993.12.01

초록

In order to maximize the riboflavin production by a mutant strain Ashbya gosspyii, the optimization of medium and fed-batch fermentation were performed. As carbon sources, glucose and soybean oil were necessary for the riboflavin overproduction. Optimal concentrations of glucose and soybean oil in the flask cultures were found to be 3.0% and 0.5%, respectively, in a complex medium containing corn steep liquor(CLS) 1%. Among the various organic nitrogen sources tested, CSL was the most effective one both for the cell growth and riboflavin overproduction.

키워드

참고문헌

  1. Economic Microbiology v.2 Vitamins Perlman,D.;A.H.Rose(ed.)
  2. Biotechnology v.4 Vitamin B₂ Pape,H.;H.J.Rehm;H.J.Rehm(ed.)G.Reed(ed.)
  3. Ind. Eng. Chem. v.42 Riboflavin by fermentation with Ashbya gossypii Pfeifer,V.F.;F.W.Tanner;D.H.Traufler
  4. J. Bacteriol. v.63 Some nutritional requirements for biosynthesis of riboflavin by Eremothecium ashbyii Hickey,R.J.
  5. Ann. Rev. Microbiol. v.26 Riboflavin oversynthesis Demain,A.L.
  6. Microbial Technology v.1 Microial process for riboflavin production Perlman,D.;H.J.Peppler(ed.);D.Perlman(ed.)
  7. Enz. Microb. Technol. v.13 Effect of growth factors on riboflavin production by Ashbya gossypii O'zbas,T;T.Kutsal
  8. Biotechnol. Appl. Biochem. v.13 Effects of agitation and aeration rates on riboflavin fermentation by Ashbya gossypii O'zbas,T.;T.Kutsal
  9. Methods of Vitamin Assay Association of vitamin chemists;M.Freed(ed.)
  10. J. Microbiol. Biotechnol. v.2 Production of L-ornithine by citrulline auxotrophic mutants of glutamate producing bacteria Choi,D.K.;W.S.Ryu;B.H.Chung;S.W.Nam;Y.H.Park
  11. Advances in Biotechnology v.3 Vitamin fermanation: B₂and $B_{12}$ Lago,B.D.;L.Kaplan;M.Moo Young(ed.);L.Vezina(ed.);K.Singh(ed.)
  12. Biotechnology v.2 Preparation of media Cejka,A.;H.Brauer(ed.)
  13. Manual of Industrial Microbiology and Biotechnolgy Substrates for large scale fermentations Miller,T.L.;B.L.Churchill;A.L.Demain(ed.);N.A.Solomon(ed.)
  14. J. Ferment. Technol. v.59 Magnesium phosphate stimulate microbial conversion of glycine into L-serine by release from regulation by ammonium ions Tanaka,Y.;H.Tanaka;S.Omura;K.Araki;K.Nakayama
  15. J. Bacteriol. v.116 Mode of action of glycine on biosynthesis of peptidoglycan Hammes,W.;K.N.Schleder;O.Kandler