Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detergent Sensitivity of mrdA and mrdB Shape-Forming Mutants of Escherichia coli

대장균의 형태형성 변이주의 계면활성제 감수성

  • Jun, Byoung-Kwon (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Park, Sung-Dae (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Lim, Si-Kyu (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Park, Wan (Department of Microbiology, College of Natural Science, Kyungpook National University)
  • 전병권 (경북대학교 자연과학대학 미생물학과) ;
  • 박성대 (경북대학교 자연과학대학 미생물학과) ;
  • 임시규 (경북대학교 자연과학대학 미생물학과) ;
  • 박완 (경북대학교 자연과학대학 미생물학과)
  • Published : 1993.10.01
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Abstract

Escherichia coli mrdAts and mrdBts mutants forming spherical cells at 42C, were employed to investigate the possible role of both inner and outer membrance structures in the determination of cell shape of gram-negative cells. Spherical cells, but not rod-shaped wild types, were specifically killed by anionic detergents, such as sarkosyl, sodium dodecylsulfate and sodium deoxycholate. From the spherical intact cells grown overnight at 42C, much more proteins were released by sakosyl.

Keywords

References

  1. J. Bacteriol. v.157 Inhibition of lateral elongation by meeillinam stimulates cell division in certain cell division conditional mutants of Escherichia coli Canepari,P.;G.Botta;G.Satta
  2. J. Bacteriol. v.145 Effects of furazlocillin, a β-lactam antibiotic which binds selectively to penicillin-binding protein 3 on Escherichia coli mutants deficient in other penicillin-binding proteins Schmidt,L.S.;G.Botta;J.T.Park
  3. J. Bacteriol. v.172 Differential effect of mutational impairment of penicillin-binding proteins 1A and 1B on Escherichia coli strains harboring thermosensitive mutations in the cell division genes ftsA, ftsQ, ftsZ;pbpB Del Portillo.F.G.;M.A.de Pedro
  4. Mol. Gen. Genet. v.200 Dispensability of either penicillin-binding protein-la or-1b involved in the essential process for cell elongation in Escherichia coli Kato,J.I.;H.Suzuki;Y.Hirota
  5. J. Bacteriol. v.145 Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation Botta,G.A.;J.T.Park
  6. Biochem. Biophys. Res. Commun. v.101 Peptidoglycan synthetic enzyme activites of highly purified penicillin-binding protein 3 in Escherichia coli:A septum-forming reaction sequence Ishino,F.;M.Matsuhashi
  7. Proc. Natl. Acad.Sci. v.74 Thermosensitive mutation in Escherichia coli simultaneously causing defects in penicillin-binding protein-1Bs and in enzyme activity for peptidoglycan synthesis in vitro Tamaki,S.;S.Nakajima;M.Matsuhashi
  8. J. Biol. Chem. v.261 Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding proteibn 2 and RodA protein Ishino,F.;W.Park;S.Tomioka;S.Tamaki;I.Takase;K.Kunugita;H.Matsuzawa;S.Asoh;T.Ohta;B.G.Spratt;M.Matsuhashi
  9. J. Bacteriol. v.175 Synthesis of peptidoglycan and membrane during the division cycle of rod-shaped. gram-negative bacteria Gally,D.;K.Bray;S.Cooper
  10. J. Bacteriol. v.171 Peptidoglycan synthesis during the cell cycle of Escherichia coli composition and mode of insertion De Jonge,B.L.M.;F.B.Wientjes;I.Jurida;F.Driehuis;J.T.M.Wouters;N.Nanninga
  11. Proc. Natl. Acad. Sci. v.82 Penicillin binding proteins:Role in initiation of murein synthesis in Escherichia coli De la Rosa,E.J.;M.A.de Pedro;D.Vazquez
  12. J. Bacteriol. v.171 Rate and topography of peptidoglycan synthesis during cell division in Escherichia coli:concept of a leading edge Wientjes,F.B.;N.Nanninga
  13. J. Bacteriol. v.154 Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12:Analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids Asoh,S.H.;Matsuzawa;M.Matsuhashi;T.Ohta
  14. J. Bacteriol. v.141 Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli Tamaki,S.;H.Matsuzawa;M.Matsuhashi
  15. J. Bacteriol. v.171 New mre genes mreC and mreD responsible for formation of the Rod shape of Escherichia coli cells Wachi,M.;M.Doi;Y.Okasa;M.Mastuhashi
  16. J. Bacteriol. v.169 Mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam and amount of penicillin-binding proteins in Escherichia coli Wachi,M.;M.Doi;S.Tamaki;W.Park;S. Nakajima-Iijima;M.Matsuhashi
  17. J. Bacteriol. v.165 Specific location of penicillin-binding proteins within the cell envelope of Escherichia coli Barbas,J.A.;J.Diaz;A. Rodriguez-teber;D.Vazquez
  18. J. Bacteriol. v.169 Membrane-murein attachment at the leading edge of the division septum:a second membrane-murein structure associated with morphogenesis of the gram-negative bacterial division septum MaAlister,T.J.;W.R.Cook;R.WeigandlL.I.Rothefield
  19. J. Bacteriol. v.136 Cell envelope and shape of Escherichia coli multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins Sonntag,I.;H.Schwarz;Y.Hirota;U.Henning
  20. J. Bacteriol. v.152 Effects of nonionic, ionic, and dipolar ionic detergents and EDTA on the Brucella cell envelope Moriyon,I.;D.T.Berman
  21. J. Bacteriol. v.115 Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate Filip,C.;G.Fletcher;J.L.Wulff;C.F.Earhart
  22. J. Bacteriol. v.146 Interaction of cations with membrane fraction of smooth and rough strains of Salmonella typhimurium and other gram-negative bacteria Stan-Lotter,H.;K.E.Sanderson
  23. Bacterial outer membrane The fusion sites between outer membrane and cytoplasmic membrane of bacteria:their role in membrane assembly and virus infection Bayer,M.E.;M.Inouye(ed.)
  24. Virology v.1 Transduction of linked genetic characters of the host by bacteriophage P1 Lennox,E.S.
  25. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowary,O.H.;N.J.Rasenberg;A.L.Farr;R.J.Randall
  26. Nature v.227 Cleavage of structured proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  27. J. Bacteriol. v.145 Citrate-tris(hydroxymethyl)aminomethane-mediated release of outer membrane sections from the cell envelope of a deep-rough (heptose-deficient lipopolysaccaride)strain of Escherichia coli O8 Irvin,R.T.;T.J.MacAlister;R.Chan;J.W.Costerton