Hyphantria cunea Nuclear Polyhedrosis Virus 봉입형의 핵다면체에 대한 단일클론 항체의 생산과 인식항원의 결정

The Production and Characterization of Monoclonal Antibodies to the Major Polyhedra Inclusion Body of the Occluded Form of Hyphantria cunea Nuclear Polyhedrosis Virus

  • 장성호 (건국대학교 이과대학 생물학과) ;
  • 홍순복 (건국대학교 이과대학 생물학과) ;
  • 이형환 (건국대학교 이과대학 생물학과) ;
  • 김종배 (축산대학 축산가공학과) ;
  • 조명환 (건국대학교 이과대학 생물학과)
  • 발행 : 1993.10.01

초록

This study was performed to produce monoclonal antibodies to the major polyhedral inclusion body (PIB) antigen of the occluded form of Hyphantria cunea nuclear polyhedrosis virus (HcNPV). PIB proteinS were purfied from the Spodoptera frugiperda cell infected with HcNPVs. Using the purified PIB protein, BALB/C mice were immunized 3 times with 2 weeks intervals. The spleen were removed and fused with Sp2/0-Ag14, mouse myeloma cells.

키워드

참고문헌

  1. Virology v.84 Baculovirus structural polypeptides Summers,M.D.;G.E.Smith
  2. Virology v.99 Infection of Spodoptera frugiperda cells with Autographa california nuclear polyhedrosis virus. Synthesis of intracellular proteins after virus infection Carstens,E.B.;S.T.Tjia;W.Doerfler
  3. Virology v.103 Proteiun synthesis in cells infected by Autographa californica Nuclear Polyhedrosis Virus(Ac-NPY):The effect of Cytosine Arabinoside Dobos,P.;M.A.Cochran
  4. Virology v.67 Degradation of matrix protein from a nuclear polyhedrosis virus Trichoplusia ni by an endogeneous protease Eppstein,D.A.;J.A.Tima;S.Y.Toung
  5. J. Gen. Virol. v.3 A possible infection pathway in the development of a nuclear polyhedrosis virus Harrap,K.A.;Robertson
  6. Virology v.50 The structure of nuclear polyhedrosis virus Harrap,K.A.
  7. Intervirol v.5 Characteristics of the nonoccluded form of a nuclear polyhedrosis virus Dougherth,E.M.;C.F.Reichelderfer;J.S.Vaughn
  8. J. virol. v.19 no.3 Occluded and Nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni:Comparative neutralization. Comparative infectivity, and In vitro growrh studies Volkman,L.;M.D.Summers;C.H.Hsieh
  9. Monoclonal Antibodies:Principles and Practice(2nd edition) Goding,J.W.
  10. Methods Enzymol. v.58 Cell fusion Kennet,R.H.
  11. Nature v.227 Cleavage of structural protein during the assembly of the head of bacteriophage T4 Laemmli,E.K.
  12. J. Immunol. Methods v.72 Immunoblotting and dot immunobiding-Current status and outlook Towbin,H.;J.Gordon
  13. Anal. Biochem. v.119 A dotimmunobinding assay for monoclonal and other antibodies Hawkes,R.;E.;Niday;J.Gordon
  14. J. Gen Virol. v.68 secretion of particles of heoatitis B-surface antigen from insect cells using a baculovirus vector Choe,Y.K.;D.H.L.Bishop;S.Y.Matsura;M.H.Choe
  15. Appl. Environ. Mico. v.36 no.1 Comparison of solid-phase radioi unoassays for baculoviruses Crawford,A.M.;P.Faulkner;J.Kalmakoff
  16. Adv. Virus.Res. v.25 The structural properties and identification of insect virus Harrap,K.A.;C.C.Payne
  17. Virology v.125 Monoclonal antibodies to Baculovirus Structural proteins:Determination of Specificities by Western Blot analysis Hohmann,A.W.;P.F.Faulkner
  18. Advan. Virus. Res. v.20 Insect viruses. Serological relationship Mazzone,H.M.;G.H.Tigner
  19. J. Virol. v.39 Application of a novel radioimmunoassay to identify cbaculovirus structural proteins that share interspecies antigenic determinants Smith,G.E.;M.D.Summers
  20. J. Virol. v.46 Molecular engineering of the Authgrapha californica nuclear polyhedrosis virus genome:Deletion mutations within the polyhedrin gene Smith,G.E.;M.J.Fraser;M.D.Summers
  21. J. Gen. Virol. v.67 Expression of the S-coded genes of lymphocytic chorimeningitis arenavirus using a baculovirus vector Matsuhara,Y.;R.D.Possee;D.H.L.Bishop
  22. J. Insect. Pathol. v.37 SDS-PAGE camparative of the NPV of M brassecae, A. californica. L. dispar Maskos,C.B.;H.G.Miltenburger
  23. J. Inverst. Pathol. v.44 Polycrylamide gel electro-phoresis of baculovirus protein:A simplified and sensitive modification for differentating between isolates Reinganum,C.
  24. Virology. v.50 The structure of nuclear polyhedrosis virus. Ⅰ:the inclusion body Harrap,K.A.
  25. J. Immunol. Methods v.73 Western and dot blotting analysis of viral antigens and antibodies:application to murine hepatitis virus Talbot,P.J.;R.L.Konbler;M.J.Buchmeier
  26. In Vitro. v.13 The establishment of two cell lines from insect Spodoptera frugiperda Vaughn,J.N.;R.H.Goodwin;G.J.Tomkins;P.McCawley
  27. Virology v.133 Neutralization of Budded Autographa california NPV by a monoclonal antibodies:Identification of the Taget Antigens Volkman,L.;A.Phyllis;Goldsmith,Roberta;P.Faulkmer
  28. Cell Culture for Biochemists, Laboratory technique in Biochemistry and Molecular Biology Adams,R.L.P.
  29. Anal Biochem. v.112 western Blotting:Electro-phoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to ummodified nitrocellulose and radiographic detection with antibody and radiodinated protein Burnette,W.N.
  30. J. Invest. Pathol. v.31 Serological study on the transmssion of a granulosis virus of the armyworm, pseudaletia unipuncta(Lepidoptera:Noctuidae) to other Lepidopterous species Chang,P.M.;Y.Tanaka
  31. Anal. Biochem. v.131 Protein blotting:Principles and applications Gershoni,J.M.;G.E.Palade
  32. Antibodies a laboratory manual Harlow;D.Lane
  33. J. Invertebr. Pathol. v.30 Propagation of Autographa californica nuclear polyhedrosis virus in cell culture:Methods for Infecting cells Hink,W.F.;E.M.Strauss;W.A.Ramoska
  34. Eur. J. Immunol. v.6 Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion Kohler,G.;C.Milstein