Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Production and Characterization of Monoclonal Antibodies to the Major Polyhedra Inclusion Body of the Occluded Form of Hyphantria cunea Nuclear Polyhedrosis Virus

Hyphantria cunea Nuclear Polyhedrosis Virus 봉입형의 핵다면체에 대한 단일클론 항체의 생산과 인식항원의 결정

  • 장성호 (건국대학교 이과대학 생물학과) ;
  • 홍순복 (건국대학교 이과대학 생물학과) ;
  • 이형환 (건국대학교 이과대학 생물학과) ;
  • 김종배 (축산대학 축산가공학과) ;
  • 조명환 (건국대학교 이과대학 생물학과)
  • Published : 1993.10.01
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Abstract

This study was performed to produce monoclonal antibodies to the major polyhedral inclusion body (PIB) antigen of the occluded form of Hyphantria cunea nuclear polyhedrosis virus (HcNPV). PIB proteinS were purfied from the Spodoptera frugiperda cell infected with HcNPVs. Using the purified PIB protein, BALB/C mice were immunized 3 times with 2 weeks intervals. The spleen were removed and fused with Sp2/0-Ag14, mouse myeloma cells.

Keywords

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