Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Agar Medium for Screening of Urease-Producing Lactic Acid Bacteria

Urease 생산 젖산균의 탐색을 위한 한천 배지

  • Published : 1993.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

An agar medium(HY) was developed to detect the urease-producing lactic acid bacteria. HY medium was prepared with the addition of tryptone, glucose and tween 80 to the supernatant of autoclaved skim milk and yeast extract mixture. There was no difference in eumeration of lactic acid bacteria between the HY and commercial media, such as M17, MRS and BCP agar. The urease activity of Streptococcus salivarius subsp. thermophilus was detected on the HY agar medium contained urea by the color change of bromocresol purple as the pH indicator, but not on the commerical agar media. Furthermore, it was succeeded to screen the urease activity of bacteria in skim milk used as a raw material in dairy product manufacture. Therefore, HY medium was proved to be suitable for the screening of urease-producing lactic acid bacteria.

Keywords

References

  1. Fed. Proc. v.31 Effects of urea hydrolysis on cell life-span and metabolism Visek,W.J.
  2. Microbiol. Rev. v.53 Microbial ureases:Significance. Regulation and Molecular characterization Mobley,H.L.T.;R.P.housinger
  3. J. Biol. Chem. v.234 The metbolism of carbon-labeled urea germ rat. Levenson,S.M.;L.V.Crowley;R.E.Horowitz;O.J.Malm
  4. Appl. Envrion, Microbiol. v.33 Urease assy and urease producing species of anerobes in the bovine rumen and human feces Wozny,M.A,;M,P.Bryant;L.V.Holdeman;W.E.C.Moore
  5. J. Diary Sci. v.57 Isolation and characteristics of a ureolytic strain of Selenomonas ruminatium. John,A.;H.R.Isaacson;M.P.Bryant
  6. Appl. Microbiol. v.28 Isolation of ureolytic Peoptostreptococcus productus from feces using d-efined medium:Failere of common urease tests Varel,V.H.;M.P.Bryant,L.V.;Holdeman;W.E.C.Moore
  7. Appl. Environ. Microbiol. v.37 Urease-producing species of intestinal anaerobes and thier activities Suzuki,K.;Y.Benno;T.Mitsuoka;S.Tanabe;K.kobashi;J.Hase
  8. Aust. J. Dairy Technol. v.37 Metabolism of Streptococcus thermophilus.2.Production of CO₂and NH₃ from urea. Tinson,W.;M.C.Broome;A.J.Hiler;G.R.Jago
  9. J. Gen. Microbiol. v.92 The elimination of urease activity in Streptococcus feacium as evidence for plasmid-coded urase Cook,A.R.
  10. J. Gen. Microbiol. v.92 Urease activity in the rumen of sheep and the isolation of urellytic bacteria Cook,A.r.
  11. Arch. Mikrobiol. v.94 Urease production and DNA-homology in the species Byfi-dobacterium suis. Matteuzzi,D.;F.Crociani
  12. Ann. Microbiol. v.133A Urease activity in the henus Bifidobacterium. Crociani,F.;D.Matteuzzi
  13. Standarsd methods for the examination of dairy products (12th ed.) American Public Health Association, INC.
  14. Concentrated starter cultures for Foods Gilliand,S.E.;S.E.GIlliand(ed.)
  15. J. Bacteriol. v.90 Damage to Streptococcus lactis resulting from growth at low PH. Harvey,R.J.
  16. J. Appl. Bacteriol. v.34 Optimum growth parameters of lactic Streotococci used for the prodcution of concentrated chees starter cultures Cogan,T.M.;D.J.Buckley;S.Condon
  17. J. Dairy Res. v.40 Some properties of frozen concentrated starters produced by continuos culture Lloyd,G.T.;E.G.Point
  18. Appl. Microbiol. v.17 Preparation of concentrated lactic Streptococcus starters Peebles,M.M.;S.E.Gilliand;M.L.Speck
  19. U.S. Patent 5,116,737 Method for growing acid-producing bacteria McCoy,D.R.