Production of Brewer's Yeast Extract by Enzymatic Method

효소 분해법에 의한 맥주효모 추출물의 제조

  • 이시경 (두산기술원 발효연구실) ;
  • 박경호 (두산기술원 발효연구실) ;
  • 백운화 (두산기술원 발효연구실) ;
  • 유주현 (연세대학교 식품공학과)
  • Published : 1993.06.01

Abstract

Cell lytic enzyme, 5'-phosphodiesterase, and AMP-deaminase were used to produce yeast extract as a natural seasoning from beer yeast cells. Prior to the addition of cell lytic enzyme, heat treatment was performed to increase the cell wall degradation` the optimum condition of the cell lytic enzyme was 50C at pH 7.0. The production yields by the enzymatic method and conventional autolysis method were 42% and 35%, respectively. The total quantity of 5'-nucleotides, GMP and IMP, produced by enzymatic method was increased by 45% than that by the conventional method. Futhermore, the operation time of enzymatic method was only 6.5 hrs, significantly reduced from 24 hrs of the conventional method.

Keywords

References

  1. J. Inst. Brew. v.93 The occurrencc of chromium in raw materials and fate in the brewing process Farre,R.;M.J.Gimeno;M.J.Lagarda
  2. J.Agri. Food Chem v.13 Amino acid composition of yeast grown different spent sulfite liquers Henry.J.P.
  3. J. Diabetes v.27 Comparison and effects of natural and synthetic glucose tolerance factor in normal and genetically diabetic mice Robert,W.T.;T.B.James;J.D.Richard
  4. J. Agri. Food Chem v.25 Preparation of chromium containing material of glucose tolerance factor activity from brewer`s yeast extract and by synthesis Edward.W.T.;M.Walter;E.R.Edward;R.W.Wayne
  5. J. Inorg. Biochem. v.21 Assay of glucose tolerance factor and its mode of action with brewer`s yeast Holdsworch,E.S.;G.Appleby
  6. 한국특허 81-1376(Rehasek. J.(Nestle)) 효모추출물의 제조방법 Rehasek. J.
  7. 한국특허 80-2743 효모엑기스의 제조방법 이철호
  8. 한국특허 83-5058 계량된 효모엑기스의 제조방법 송인상
  9. Japan Patent 54-14176 ビ-ル酵母エキスの製造法 Ajinomoto
  10. Japan Patent 57-71376 酵母エキスの製造方法 Ajinomoto
  11. Japan Patent 51-32766 酵母エキスの製造方法 出光興産
  12. Japan Patent 54-28467 酵母エキスの製造方法 出光興産
  13. U.S. Patent 3961080 Process for autolusis of yeast Kikkoman
  14. Japan Patent 54-13496 ビ-ル酵母エキスの製造法 Kikkoman
  15. J. Food Sci. v.44 An enzymatic method for yeast autolysis Knorr.D.;K.J.Shetty;L.F.Hood;J.E.Kinsella
  16. Process Biochemistry Yeast autolysis Hough,J.S.;I.S.Maddix
  17. 한국식품과학회지 v.13 자기소화시간에 따른 효모 extract의 성분과 풍미변화 이철호;박장열;정경식
  18. U.S. Patent 4264628 Process for the production of a yeast autolysis Henkel
  19. U.S. Patent 42985976 Method for accelerating autolysis of yeast Standard Oil Co.
  20. ビ-ル酵母の驚異 赤澤好溫
  21. 日本食品工業 no.4月(下) 富田剛
  22. Wallerstein Labs Communs v.7 Brewer`s yeast Donough, M.J.V.;T.C.Haffenreffer
  23. Brewer` Digest v.29 Brewer`s yeast. washing and debettering Nay,E.V.
  24. J. Ferment. Technol. v.43 Digestion of yeast cells by yeast cell wall lytic enzyme Tabata,S.;G.Terui
  25. Eur. J. Biochem. v.64 Lysis of yeast cell wall Rombouts,F.M.;H.J.Phaff
  26. J. Food Sci. v.39 Synergistic effect of ethanol and sodium chroride on autolysis baker`s yeast for preparing food grade yeast extract Hirosh. S.