한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제21권3호
  • /
  • Pages.263-268
  • /
  • 1993
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

치면세균막 분해효소인 $\alpha$-1,3 glucanase를 생산하는 미생물의 분리 및 효소 특성

Isolation of $\alpha$-1,3 Glucanase from Microorganism and the Prodution of High Activity $\alpha$-1,3 Glucanase for Hydrolysis of Dental Plaque

  • 조효상 (수원대학교 공과대학 유전공학과) ;
  • 허태련 (인하대학교 공과대학 생물공학과) ;
  • 윤정원 (수원대학교 공과대학 유전공학과)
  • 발행 : 1993.06.01
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초록

Seventeen strains were isolated from soil, cattle rumen, cereal sewage dregs, insect on agar plate containing insoluble glucan as a sole carbon source from immobilized Streptococcus mutans, which produced alpha-1,3 glucanase for lysis of dental plaque. Among these strains isolated from soil, SW-522 and SW-713 that had appeared to produce the high level of alpha-1,3 glucanase, degraded insoluble glucan from S. mutans 97.6% and 49.4%, respectively in 5 hours. The activity of crude alpha-1,3 glucanase from SW-522 was 1.3mg insoluble glucan/min.mg protein. This enzyme was entirely degraded insoluble glucan on glass tube which produced by S. mutans in TH medium with 5% sucrose.

키워드

참고문헌

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