한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제21권3호
  • /
  • Pages.256-262
  • /
  • 1993
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Bacillus acidocaldarius가 생산하는 Cyclodextrin Glycosyltransferase의 특성

Characterization of the Cyclodextrin Glycosyltransferase Produced by Bacillus acidocaldarius

  • 발행 : 1993.06.01
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초록

Nine novel cyclodextrin glycosytransferase-producing bacteria were isolated from soil in a low acidic pH (3-4) medium at high temperature (45-60C). The isolated acidophilic bacteria were identified as Bacillus acidocaldarius. Highest yield of enzyme was obtained by using the following medium: 4% raw potato, 1% peptone, 0.1% yeast extract, 0.02% (NH4)2SO4, 0.05% MgSO4, 0.02% CaCl2, 0.3% KH2PO4. The crude enzyme showed a very broad pH-activity curve and had two optium pH ranges at 30 and 5.0-6.0. The crude enzyme was most active at 90C.

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참고문헌

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