Lactobacillus helveticus의 Protoplast 형성과 재생에 관한 연구

Protoplast Formation and Regeneration in Lactobacillus helveticus

  • 전홍기 (부산대학교 자연과학대학 미생물학과) ;
  • 박현정 (부산대학교 자연과학대학 미생물학과) ;
  • 백형석 (부산대학교 자연과학대학 미생물학과) ;
  • 송재철 (울산대학교 자연과학대학 식품영양학과)
  • 발행 : 1993.04.01

초록

The optimal conditions for the production and regeneration of L. helveticus protoplasts were examined. The protoplast formation of L. helveticus was most efficient obtained when the cells grown to mid and late logarithmic phase in MRS medium were used. The maximum number of protoplasts was obtained when lysozyme and mutanolysin were used to lysis the cell wall in 20mM HEPES buffer (pH 7.0) containing 1M sucrose. Regeneration was accomplished with a complex medium containing 10% sucrose, 10mM MaCl2, 20mM CaCl2, 5% gelatin and 0.5% bovine serum albumin. The regeneration frequency of the protoplasts was 10-20% after 5 days of incubation at 30C.

키워드

참고문헌

  1. Int. J. Syst. Bacteriol. v.20 Characters used in the classification of lactobacilli Rogosa,M.
  2. Agric. Biol. Chem. v.48 Lipo-some-mediated transfection of Lactobacillus casei spheroplasts Shimizu-Kadota,M.;S.Kudo
  3. Proc. Natl. Acad. Sci. USA v.73 Fusion of bacterial protoplasts Schaeffer,P.;B.Cami;R.D.Hotchkiss
  4. Agric. Biol. Chem. v.47 Protoplast formation and regeneration of Streptococcus lactis cells Okamoto,T.;Y.Fujita;R.Irie
  5. Appl. Environ. Microbiol. v.48 Production and regeneration of Lactobacillus casei protoplasts Lee-Wickner,L.J.;B.M.Chassy
  6. J. Appl. Bacteriol. v.23 A medium for the cultivation of lactobacilli De Man,J.C.;M.Rogosa;M.E.Sharpe
  7. J. Bacteriol. v.116 Factors affecting the resistance of Lactobacillus fermenti to lysozyme Neujahr,H.Y.;B.Borstad;I.M.Logardt
  8. Appl. Environ. Microbiol. v.39 Method for the lysis of gram-positive, asporogenous bacteria with lysozyme Chassy,B.M.;A.Giuffrida
  9. Appl. Environ. Microbiol. v.43 Transformation of Streptococcus lactis protoplasts by plasmid DNA Kondo,J.K.;L.L.Mckay
  10. Kor. J. Appl. Microbiol. Bioeng. v.14 A study on the protoplast formation and regeneration of Lactobacillus casei YIT 9018 Baek,Y.J.;H.S.Bae;M.Yoo;Y.K.Kim;H.U.Kim
  11. J. Gen. Microbiol. v.107 Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration Baltz,R.H.
  12. Kor. J. Food Sci. Technol. v.16 Protoplast formation and regeneration of Streptococcus lactis Cha,S.H.;W.C.Shin;D.H.Oh;J.H.Yu
  13. Agric. Biol. Chem. v.43 Fusion of protoplasts and genetic recombination of Brevibacterium flavum Kaneko,H.;K.Sakaguchi
  14. Agric. Biol. Chem. v.45 Studies on regeneration media for Bacillus subtilis protoplasts Akamatsu,T.;J.Sekiguchi
  15. Agric. Biol. Chem. v.53 Protoplast formation and regeneration of Lactobacillus plantarum Kanatani,K.;H.Miura;M.Sakamoto;M.Oshimura
  16. Kor. J. Appl. Microbiol. Bioeng. v.13 Regeneration of yeast protoplast in Hansenula anomala var. anomala and Saccharomyces cerevisiae Koo,Y.J.;W.S.Park;D.H.Shin;T.J.Yu
  17. Agric. Biol. Chem. v.45 A modified regeneration method for streptomycete protoplasts Shirahama,T.;T.Furumai;M.Okanishi
  18. Appl. Environ. Microbiol. v.44 Optimal cultural and physiological conditions for handling Streptomyces rimosus protoplasts Pigac,J.;D.Hranueli;T.Smokvina;M.Alacevic