미생물에 의한 폐수중의 아조계 염료의 분해

Decolourization of Azo Dyes in Effluents by Klebsiella Owtoca

A research was performed to improve the treatment of waste water from dyeing factories by biological method. Some strains were separated from activated sludge and the superior strain which decolourize some auto dyes was used for this study. The strain was cultivated on two type of culture medium that contains three azo dyes, and the characteristics of strain was investigated. The separated superior strain was identified to Klebsiella oxytoca classified as gram-negative facultatively anaerobic rods in Bergey's manual, and decolourization rate of three azo dyes was discussed. Dye concentration decreased as strain grew. Dyes in nutrient sufficient culture medium was decclourlzed by Klebsiella oxyteca faster than those in nitrogen limited culture medium. As the evidence of transformation of strain, plasmid were separated from Klebsiella oxytoca. From IR spectra of precipitates in medium after culture, it could be assumed that the mechanisms of decolourization were deformation or adsorption of dyes by micro-organism.