Hybridoma배양을 위한 무혈청 배지의 개발 제2부 : 무혈청 배지를 사용한 Hybridoma배양

Development of Serum-Free Medium for Mouse-mouse Hybridoma Part II. Hybridoma Culture using Developed Serum-Free Media

The serum free medium was developed and used for the suspension culture of mammalian cells. Although there were the problems of the longer lag time and the smaller maximum cell concentration achievable, the higher specific productivity as well as other advantages of the serum free medium can make it a more realistic alternative. The existence of a staggering period in glucose concentration vs. time profile in the batch culture can be a practical indicating signal for performing fed batch culture. The concentration dependence of the effects of the additives in the serum free medium as well as its economic feasibility was also tested.