미생물학회지 (Korean Journal of Microbiology)
- 제31권1호
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- Pages.1-8
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- 1993
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
핵전이에 의한 Aspergillus niger와 Penicillium verruculosum F-3의 속간 잡종형성
Formation of Intergeneric Hybrids Between Aspergillus niger and Penicillium verruculosum by Nuclear Transfer
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양영기
(조선대학교 자연과학대학 유전공학과) ;
-
박열
(조선대학교 자연과학대학 유전공학과) ;
- 김성준 (조선대학교 자연과학대학 유전공학과) ;
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정현숙
(조선대학교 자연과학대학 유전공학과) ;
- 임채영 (조선대학교 자연과학대학 유전공학과) ;
-
이영하
(충남대학교 자연과학대학 미생물학과)
-
Yang, Young-Ki
(Department of Genetic Engineering, Chosun University) ;
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Park, Yeol
(Department of Genetic Engineering, Chosun University) ;
- Kim, Sung-Joon (Department of Genetic Engineering, Chosun University) ;
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Cheong, Hyeon-Suk
(Department of Genetic Engineering, Chosun University) ;
- Lim, Chae-Young (Department of Genetic Engineering, Chosun University) ;
-
Rhee, Young-Ha
(Department of Microbiology, Chungnam National University)
- 발행 : 1993.02.01
초록
Aspergillus niger 와 Penicillium verruculosum F-3 균주의 돌연변이체 선발은 ultraviolet (UV) 단일처리와 UV 및 N-methyl-N'-nitrosoguanidine(MNNG) 이중처리에 의해 얻었다. 원형질체 형성을 위한 최적조건으로는 pH 5.8, 상투압안정제로는 0.6 M KCI이 최적이었으며, A-niger 와 P. verruculosum 의 재생율은 각각 54.6%, 49.8% 로 나타났다. 핵전이에 의한 형질전환율은
lntergeneric hybrids formed between Aspergillus niger and Penicillium verruculosum were obtained by nuclear transfer technique. Nuclei isolated from wild type and auxotrophic mutants of donor strains were transferred into the protoplasts of different auxotrophic mutants as recipient strains. Several auxotrophic mutants were isolated from conidiospores of the two strains mutagenized with ultraviolet and N-methyl-N'-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of intergeneric hybrid formation by nuclear transfer were