Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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REGULATION OF PROENKEPHALIN GENE EXPRESSION AND MET-ENKEPHALIN SECRETION IN BOVINE ADRENAL MEDULLARY CHROMAFFIN CELLS AND C6 RAT GLIOMA CELLS

  • Suh, Hong-Won (Department of Life Science, Kwangju Institute of Science and Technology)
  • Published : 1993.12.01
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Abstract

The expression of proenkephalin (proENK) mRNA and Met-enkephalin (ME) secretion in C6 rat glioma cells and bovine adrenal medullary chromaffin (BAMC) cells were elucidated in the present study. The levels of proENK mRNA and ME secreted into the media in BAMC cells were measured in the presence of cycloheximide and 12-tetrade-canoylphorbol-13-acetate (TPA). Cycloheximide (20 nM) abolished the induction of proENK mRNA expression, protein synthesis and ME secretion by TPA (1nM), indicating that de novo protein synthesis was necessary for proENK gene expression and ME secretion.

Keywords

References

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