Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A STUDY ON ANTIGENICITY OF RECOMBINANT HUMAN GROWTH HORMONE (LBD-007) IN MICE AND GUINEA PIGS

  • Park, Jong-Il (Toxicology Center Korea Research Institute of Chemical) ;
  • Han, Sang-Seop (Toxicology Center Korea Research Institute of Chemical) ;
  • Roh, Jung-Koo (Toxicology Center Korea Research Institute of Chemical)
  • Published : 1993.06.01
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Abstract

Antigenic potential of recombinant human growth hormone (LBD-007), a newly developed drug for growth hormone deficiency, was investigated in mice and guinea pigs. 1. Mice showed production of antibodies against LBD-009 (1.5IU/kg) with aluminum hydroxide gel(alum) as an adjuvant, Judaged by the heterologous anaphylaxis (PCA) test using rats. On the other hand, antibodies against ovalbumin (OVA) inoculated with alum were definitely detected. 2. In the studies with guinea pigs, both the inoculation of LBD-009 (0.15IU/kg-1.5IU/kg) only and of LBD-009 with complete Freund's adjuvant (CFA) as an adjuvant did produce weak positive reactions in homologous passive cutaneous anaphylaxis (PCA). On the other hand, the inoculation of ovalbumin with complete Freund's adjuvant (CFA) produced positive reaction in PCA.

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References

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