한국동물학회지 (The Korean Journal of Zoology)

한국통합생물학회 (The Korean Society for Integrative Biology)
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사람 alpha-fetoprotein에 대한 단일클론 항체의 생산 및 분석

Production and Characterization of anti-AFP Monoclonal Antibodies

  • Kang, Hee-Kap (Department of Biology, College of Notural Science, Hyungpook National University, Taegu 702-701) ;
  • Tae (Department of Biology, College of Notural Science, Hyungpook National University, Taegu 702-701)
  • 발행 : 1993.10.01
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초록

Monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFPI was produced by hybridizing SP 210-Ag 14 mouse myeloma cells with spleen cells of Balb/c mice immunized with purified AFP. Two subclones (D-6 and I-6) were expanded as ascite tumors in svngenic mice, and from ascitic fluid immunoglbulins were Pruified. Each anibodv was identified to be homogeneous by several criteria, and the affinity constant of D-6 and I-6 MAb to AEP was calculated to be 4.2${\times}$10-8 and 6.4${\times}$10-8 M-1, respectively. With these MAbs sensitive and accurate enzyme linked immunosorbent assay method was established.

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