한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제17권3호
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- Pages.263-267
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- 1993
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- 1226-5284(pISSN)
유전자 변환 동물 생산을 위한 Plasmid Vector(pSS4)의 개발
Development of a Plasmid Vector(pSS4) for the Use in Animal Transgenesis
초록
Transgenic animals have become an important tool in the basic and applied sectors of genetic and biomedical sciences. In particular transgenes provide clear-cut markers in the spatial and temporal analysis of developing embryos for the understanding of developmental mechanisms. For the long-term use of plasmid vector in a particular purpose it would be necessary to develop one's own vector system which can be properly expressed in eukaryotic system. Plasmids were constructed from ori region of pUC19 and early region of SV40 through various steps. LacZ gene coding for
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