Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
권호 표지

Preparation of Living Skin Equivalent by using the Contracted Collagen Lattice and Cultured Human Keratinocytes

수축된 콜라겐 격자와 배양된 각질형성세포를 이용한 피부 대용물질의 제조에 관한 연구

  • Park, Jae-Gyeong (Laboratory of Immunology, Kyung Hee University) ;
  • Jo, Geum-Cheol (Department of General Surgery, Kyung Hee University Hospital) ;
  • Park, Ho-Cheol (Department of General Surgery, Kyung Hee University Hospital)
  • 박재경 (경희대학교 의과대학 부속병원 면역학연구실) ;
  • 조금철 (경희대학교 의과대학 부속병원 외과학교실) ;
  • 박호철 (경희대학교 의과대학 부속병원 외과학교실)
  • Published : 1993.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

An experimental study was performed for the preparation of living skin-equivalent by the using collagen gel contraction with human fibroblasts as neodermls and cultured human keratinocytes as neoderm is . The results were as follows ; 1) The rate of collagen gel contraction was dependent on the number of fibroblasts into the lattice and collagen contraction was progressed according to the increment of the number of the cells. 2) The rate of collagen gel contraction was progressed according to the decrement of the contraction of the collagen. 3) The rate of gel contraction was progressed according to the increment of serum concentration in the fixed concentration of the fibroblasts and collagen. 4) The lattice contraction was decreased according to the increment of the population doublings of the fibroblasts. 5) Macroscopically, the artificial dermis was gray white in color and tissue-like consistency and elas- ticity. 6) Microscopically, three dimensionally contracted artificial dermis showed more dense fibroblasts and its newly formed collagen fibrils in the matrix than one dimensionally contracted one. 7) Finally prepared skin-equivalent showed good attachment of living stratified keratinocytes to the dermal equivalent microscopically. It has been proposed that newly formed skin-equivalent is suitable for the graft of extensively and deeply burned patients. Shortening of the manufacturing period of skin-equivalent and development of conservation technique as a readily usable state are to be solved for our ongoing works.

Keywords

References

  1. Proc Natl Acad Sci USA v.76 Production of tissue like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro Bell,E.;Ivarrson,B.;Merrill,C.
  2. J Cell Biol v.83 Characteristics of a tissue equivalent formed by fibroblasts cast in a collagen gel Bell,E.;Merrill,C.;Solomon,D.
  3. J Invest Dermatol v.81 The reconstruction of living skin Bell,E.;Sher,S.;Hull,B.;Merrill,C.;Rosen,S.;Charson,A.;Asselinean,D.;Dubertret,L.;Coulomb,B.;Lapiere,C.;Nusgens,B.;Neveux,Y.
  4. Ann Surg v.144 Studies on wound healing with special reference to the phenomenon of contraction in experimental wounds in rabbit skin Billingerham,R.E.;Russel,P.S.
  5. Ann Surg v.194 Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury Burke,J.F.;Yannas,I.V.;Quinby,W.C.Jr;Bondoc,C.C.;Jung,W.K.
  6. Plast Reconstr Surg v.30 Synthetic substitute for skin Chardak,W.M.;Martin,M.M.;Jewett,T.C.;Boyer,B.E.
  7. Burns v.6 The intermingled transplantation of auto-and homografts in severe burns Chih-Chun,Y.;Tsi-Siang,S.;Te-An,C.
  8. Int Rev Conn Tis Res. v.6 Medical and surgical applications of collagen Chvapil,M.;Koronenthal,R.L.;Vanwinkle,W.
  9. J Invest Dermatol. v.92 Influence of human dermal fibroblasts on epidermalization Coloumb,B.;Leberton,C.;Dubertret,L.
  10. J Biomed Mater Res. v.14 Design of artificial skin:part Ⅲ, Control of pore structure Dagalakis,N.;Flink,J.;Stasikelis,P.
  11. Burns v.10 Synthetic materials for covering burn wounds:Progress towards perfection. Part Ⅰ, Short-term dressing materials Davies,J.W.L.
  12. Burns v.10 Synthetic materials for covering burn wounds:Progress towards perfection. Part Ⅱ, Longer-term substitutes for skin Davies,J.W.L.
  13. Clin Immunol & Immunopathol v.38 Loss of allogeneic T cell activating ability and Langerhans cell markers in human epidermal cell culture Demidem,A.;Faure,M.;Dezutter-Dambuyant,C.;Thivolet,J.
  14. Science v.211 Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness Ehrich,P.H.;Buttle,D.J.;Nakatsuji,T.
  15. Proc Natl Acad Sci USA v.76 Inproduction of tissue like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro Ehrmann,R.L.;Gey,G.O.
  16. J Cell Biol v.54 Role of human fibroblasts in collagen contraction Elsdale,T.;Bard,J.
  17. N Engl J Med. v.311 Permanent coverage of large burn wounds with autologous cultured human epithelium Gallico,C.G.;O'Connor,N.E.;Campton,C.C.;Kehinde,O.;Green,H.
  18. Transplantation v.2 The acceptance and evolution of dermal homografts freed of viable cells Grillo,H.C.;Mckhann,C.F.
  19. Arch dermatol. v.119 Injectable collagen implants Hanke,C.W.;Robinson,J.K.
  20. Lancet v.2 Grafting of burn patients with allografts of cultured epidermal cells Hefton,J.M.;Madden,M.R.;Finkelstein,J.L.;Shires,G.T.
  21. Biochemical methods in cell culture and virology(1st ed.) Kuchler,R.J.
  22. J Invest Dermatol. v.78 Cultured human epithelial cells do not synhesize HLA-DR Morhenn,V.B.;Benike,C.J.;Cox,A.J.;Charron,D.J.;Engleman,E.G.
  23. Lancet v.1 Grafting of burns with cultured epithelium prepared from autologous epidermal cells O'Connor,N.E.;Mulliken,J.B.;Banks-Schlegel,S.;Kehinde,O.;Green,H.
  24. J Kor Surg Soc v.37 no.2 In vitro culture of human keratinocytes and perspectives on their use for grafting of patients with extensive burns Park,H.C.;Oh,S.M.;Joo,H.Z.;Park,J.K.;Choi,Y.M.
  25. J Trauma v.27 Biosynthetic skin substitute versus frozen human cadaver allograft for temporary coverage of excised burn wounds Prude,G.F.;Hunt,J.L.;Gillespis,R.W.
  26. Arch Dermatol. v.120 Intradermal implantation of bovine collagen; Human immune responses associated with clinical reactions Siegel,R.J.;McCoy,J.P.;Schade,W.;Swanson,N.A.
  27. Transplantation v.36 Acceptance of allogeneic fibroblasts in skin equivalent transplants Sher,S.E.;Hull,B.;Rosen,S.;Church,D.;Friedman,L.;Bell,E.
  28. J Biomed Mater Res. v.14 Design of an artificial skin; Ⅰ. Basic design principles Yannas,I.V.;Burke,J.F.