약학회지 (YAKHAK HOEJI)

  • 제37권6호
  • /
  • Pages.598-604
  • /
  • 1993
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

능소화의 꽃받침으로부터 Protein Kinase C 저해물질인 Verbascoside의 분리 및 그 생물활성

Isolation and Biological Activity of Verbascoside, A Potent Inhibitor of Protein Kinase C from the Calyx of Campsis grandiflora

  • 이현선 (한국과학기술연구원 유전공학연구소) ;
  • 박문수 (한국과학기술연구원 유전공학연구소) ;
  • 오원근 (한국과학기술연구원 유전공학연구소) ;
  • 안순철 (한국과학기술연구원 유전공학연구소) ;
  • 김보연 (한국과학기술연구원 유전공학연구소) ;
  • 김환묵 (한국과학기술연구원 유전공학연구소) ;
  • 오구택 (한국과학기술연구원 유전공학연구소) ;
  • 민태익 (한국과학기술연구원 유전공학연구소) ;
  • 안종석 (한국과학기술연구원 유전공학연구소)
  • 발행 : 1993.12.01
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초록

The calyx extract of Campsis grandiflora displayed inhibitory activity against protein kinase C from the bovine brain. Separation guided by protein kinase C enzyme assay and bleb forming assay led to isolation of a potent protein kinase C inhibitor that was identified as a known phenylpropanoid glycoside, verbascoside. It suppressed completely bleb-formation of K562 cell surface induced by phorbol 12,13-dibutylate at the concentration of 60 $\mu\textrm{g}$/ml and IC$_{50}$ of the protein kinase C occured at 20 $\mu{M}$. This compound was tested for cytotoxic activity against ten human tumor cell lines in vitro. it exhibited moderate cytotoxic activity against skin tumor cell line M14 (IC$_{50}$ 2.2 $\mu\textrm{g}$/ml) and very weak cytotoxicity against other cell lines (IC$_{50}$>10 $\mu\textrm{g}$/ml)

키워드

참고문헌

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