YAKHAK HOEJI (약학회지)
- Volume 37 Issue 5
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- Pages.504-510
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- 1993
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Molecular Cloning And analysis of Korean Insulin Gene
한국인 인슈린 유전자의 클로닝 및 분석
Abstract
Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3'-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5'-regulatory region(G2101
Keywords
- Insulin;
- insulin dependent diabetes mellitus(IDDM);
- non-insulin dependent diabetes mellitus(NIDDM);
- restriction fragment length of polymorphism(RFLP)