약학회지 (YAKHAK HOEJI)
- 제37권2호
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- Pages.149-157
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- 1993
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
돼지 간장 조직에서 얻은 단백질 메칠라제 저해제의 정제와 특성
Protein Methylase Inhibitor from Porcine Liver : Purification and Properties
초록
Protein methylase inhibitor which is a modulator of biological methylation has been purified and characterized from porcine liver soluble fraction by cell fractionation, Sephadex G25 chromatography, reverse phase HPLC, size exclusion HPLC. The results are summarized as follows. 1) The purified inhibitor shows apparent homogeneity, as judged by HPLC. 2) A molecular weight of the purified inhibitor which is composed of 18 amino acid residues is about 1,400 daltons. 3) A single absorption peak of ultraviolet spectrum was observed at 260nm. 4) The inhibitor was not inactivated by heating at
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