Parasites, Hosts and Diseases
- 제31권1호
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- Pages.49-56
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- 1993
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
유구낭미충증의 혈청학적 진단을 위한 ABC-ELISA와 Protein A-ELISA의 유용성
Applicability of ABC-ELISA and Protein A-ELISA in serological diagnosis of cysticercosis
- Lee, Jong-Hyun (Department of Parasitology, College of Medicine, Chung-Ang University) ;
- Kong, Yoon (Department of Parasitology, College of Medicine, Chung-Ang University) ;
- Ryu, Jae-Young (Department of Parasitology, College of Medicine, Chung-Ang University) ;
- Cho, Seung-Yull (Department of Parasitology, College of Medicine, Chung-Ang University)
- 발행 : 1993.03.01
초록
현재 유구낭미충증을 혈청학적으로 진단하는 데에는 낭액항원을 이용한 특이항체검사법으로 micro-ELISA를 널리 이용하고 있다. 이 실험은 효소부착 이차항체를 사용하는 micro-ELISA방법 대신 민감도가 뛰어난 것으로 알려진 ABC-ELISA나 Protein-ELISA로 바꾸어 사용하면 검사의 민감도를 보다 개선할 수 있는지를 알기 위하여 실시하였다. ABC-ELISA에 의한 항체검사는 낭액항원 단백질
Specific antibody test in serum and cerebrosinal fluid (CSF) is still the main mode of serological diagnosis of cystiercosis. Of different techniques of artibody test, enzyme-linked immunosorbent assay (micro-ELISA) has widely been applied. This study was undertaken to observe whether diagnostic capability can be Improved by applying more sensitive techniques such as Protein A-ELISA and avidin biotin complex ELISA (ABC-ELISA). When evaluated using 115 sera of human cysticercosis, the antibody positive rates were not significantly improved in Protein A-ELISA (82.6%) and in ABC-ELISA (86.1%) than in micro-ELISA (81.7%). The specificities, evaluated in 165 sera from other diseases and normal controls, were significantly improved (88.5% by micro-ELISA, 93.3% by Protein A-ELISA and 93.8% by ABC-ELISA). Antibody levels (absorbance, abs.) in individual serum were correlated well (r : 0.83∼0.86) each other. An actual benefit of Protein A-ELISA and ABC-ELISA was that they needed smaller amount of test sample.