Parasites, Hosts and Diseases
- Volume 31 Issue 1
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- Pages.49-56
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- 1993
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Applicability of ABC-ELISA and Protein A-ELISA in serological diagnosis of cysticercosis
유구낭미충증의 혈청학적 진단을 위한 ABC-ELISA와 Protein A-ELISA의 유용성
- Lee, Jong-Hyun (Department of Parasitology, College of Medicine, Chung-Ang University) ;
- Kong, Yoon (Department of Parasitology, College of Medicine, Chung-Ang University) ;
- Ryu, Jae-Young (Department of Parasitology, College of Medicine, Chung-Ang University) ;
- Cho, Seung-Yull (Department of Parasitology, College of Medicine, Chung-Ang University)
- Published : 1993.03.01
Abstract
Specific antibody test in serum and cerebrosinal fluid (CSF) is still the main mode of serological diagnosis of cystiercosis. Of different techniques of artibody test, enzyme-linked immunosorbent assay (micro-ELISA) has widely been applied. This study was undertaken to observe whether diagnostic capability can be Improved by applying more sensitive techniques such as Protein A-ELISA and avidin biotin complex ELISA (ABC-ELISA). When evaluated using 115 sera of human cysticercosis, the antibody positive rates were not significantly improved in Protein A-ELISA (82.6%) and in ABC-ELISA (86.1%) than in micro-ELISA (81.7%). The specificities, evaluated in 165 sera from other diseases and normal controls, were significantly improved (88.5% by micro-ELISA, 93.3% by Protein A-ELISA and 93.8% by ABC-ELISA). Antibody levels (absorbance, abs.) in individual serum were correlated well (r : 0.83∼0.86) each other. An actual benefit of Protein A-ELISA and ABC-ELISA was that they needed smaller amount of test sample.
현재 유구낭미충증을 혈청학적으로 진단하는 데에는 낭액항원을 이용한 특이항체검사법으로 micro-ELISA를 널리 이용하고 있다. 이 실험은 효소부착 이차항체를 사용하는 micro-ELISA방법 대신 민감도가 뛰어난 것으로 알려진 ABC-ELISA나 Protein-ELISA로 바꾸어 사용하면 검사의 민감도를 보다 개선할 수 있는지를 알기 위하여 실시하였다. ABC-ELISA에 의한 항체검사는 낭액항원 단백질