Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Brassinosteroid substances in immature oryza sativa seeds

벼종자의 brassinosteroid 활성물질

  • Park, Keun-Hyung (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Kim, Seon-Jae (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Park, Jong-Dae (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Lee, Lan-Sook (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Hyun, Kyu-Hawn (Department of Resources Plant, College of Agriculture, Sunchon University)
  • 박근형 (전남대학교 농과대학 식품공학과) ;
  • 김선재 (전남대학교 농과대학 식품공학과) ;
  • 박종대 (전남대학교 농과대학 식품공학과) ;
  • 이란숙 (전남대학교 농과대학 식품공학과) ;
  • 현규환 (순천대학교 농과대학 자원식물학과)
  • Published : 1993.10.31
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Abstract

To investigate the presence of the brassinosteroid substances in immature Oryza sativa L. cv Tongjinbyeo seeds, the methanol extract was purified by the sequential use of solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 chromatography and charcoal adsorption chromatography. The activity of brassinosteroid was monitored by the rice inclination test and its presence could be confirmed in each purification step. The purified active components were separated by silica gel adsorption chromatography and Sephadex LH-20 chromatography. Brassinosteroid substances in separated active fractions were identified as castasterone, teasterone and 6-deoxocastasterone by HPLC. Our work is probably the first report of endogenous brassinosteroids in Oryza sativa seeds. The content of brassinosteroid in Oryza sativa seeds as converted into brassinolide was $0.5{\sim}1.5\;ng/g$ fresh weight.

벼종자에 존재하는 brassinosteroid 활성물질을 탐색하기 위하여 동진벼 미숙종자를 MeOH로 추출하고, 이 추출물을 rice inclination test를 지표로, 용매분획, silica gel adsorption chromatography, Sephadex LH-20 chromatography, charcoal adsorption chromatography 등으로 brassinosteroid 활성물질을 정제한 다음, silica gel 흡착 chromatography로 활성성분을 분리하였다. HPLC에 의해 활성본체를 구명한 결과, castaserone, teasterone, 6-deoxocastasterone이 최초로 동정되었다. 벼종자에 함유된 brassinosteroid 활성물질의 함량은 brassinolide로 환산하여 생체중량 g당 $0.5{\sim}1.5\;ng$ 수준이었다.

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