Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Identification of the growth stimulatory components in the single and mixed cultures of Propionibacterium freudenreichii KFCC 31227 and Lactobaciilus acidophilus KFCC 32825

Propionibacterium freudenreichii KFCC 31227과 Lactobacillus acidophilus KFCC 32825의 단독 및 혼합배양에 의한 영양성분의 상호작용

  • Min, Yun-Sik (Department of Food Engineering, Chungiu National University,Department of Food Engineering Chungbuk National University) ;
  • Kim, Ki-Cheul (Department of Food Engineering, Chungiu National University,Department of Food Engineering Chungbuk National University) ;
  • Lee, Myong-Yur (Department of Food Engineering, Chungiu National University,Department of Food Engineering Chungbuk National University) ;
  • Lee, Ung-Soo (Department of Food Engineering, Chungiu National University,Department of Food Engineering Chungbuk National University)
  • 민윤식 (충주산업대학교 식품공학과,충북대학교 식품공학과) ;
  • 김기철 (충주산업대학교 식품공학과,충북대학교 식품공학과) ;
  • 이명열 (충주산업대학교 식품공학과,충북대학교 식품공학과) ;
  • 이응수 (충주산업대학교 식품공학과,충북대학교 식품공학과)
  • Published : 1993.10.31
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Abstract

The effects of culture broth filtrates, sugar changes and utilizationon the growth and acid production of P. freudenreichii KFCC 31227and L. acidophilus KFCC 32825 were investigated in S.G.Y.(Skim milk, glucose, yeast extract) and SMW (skin milk whey)medium by the single and mixed cultures. The growth and acid production by mixed culture and in cultured broth filtrate of the other party were more affected than those of single culture and self-cultured broth filtrate. When the two strains were cultured, P. freudenreichii KFCC 31227 utilized with lactose more than glucose and L. acidophilus KFCC 32825 was glucose more than lactose in the growth and acid production. The mixed culture of two strains was more affected to sugar utilization than single culture. This result was considered due to the synergistic effect by interaction of these two strains in mixed culture.

S.G.Y.배지(skim milk, glucose, yeast extract)와 S.M.W.배지(skim milk whey). 배지에 P. freudenreichii KFCC 31227과 L. acidophilus KFCC 32825를 단독 또는 혼합배양 후, 두 균주의 생육 및 유기산 생성에 미치는 발효여액의 영향과 당의 변화 및 이용 효과를 검토한 결과, 자신의 발효여액보다는 상대편의 발효여액에서 그리고 단독배양보다는 혼합배양에서 생육이나 산 생성의 효과가 촉진되었으며, 배지중의 glucose 및 lactose 의 이용효과를 보면 P. freudenreich-ii KFCC 31227 단독 배양시는 lactose 를, L. acdophilus KFCC 32825 단독배양시는 glucose를 생육이나 산 생성에 더 잘 이용하였다. 또한 두 균주의 혼합배양시에는 단독배양에 비해 발효에 glucose 나 lactose 를 이용하는 시간이 더욱 단축되는 결과로 나타났으며, 이것은 혼합배양시 두 균주의 상호작용에 의한 synergism 효과에 기인된 것으로 생각된다.

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