Applied Biological Chemistry
- Volume 36 Issue 3
- /
- Pages.158-162
- /
- 1993
- /
- 2468-0834(pISSN)
- /
- 2468-0842(eISSN)
Properties of a bilirubin oxidase from Penicillium sp. LAM 91-89
Penicillium sp. LAM 91-89가 생산하는 bilirubin oxidase의 특성
-
Yi, Dong-Heui
(Department of Microbiological Technology, Kon-Kuk University) ;
- Lee, Dong-Ho (Department of Microbiological Technology, Kon-Kuk University) ;
- Kim, Jung-Bae (Department of Microbiological Technology, Kon-Kuk University) ;
- Lee, No-Woon (Department of Microbiological Technology, Kon-Kuk University)
- Published : 1993.06.30
Abstract
A bilirubin oxidase produced by Penicillium sp. strain LAM 91-89 was purified and partially characterized. The enzyme was purified about 70 folds from culture broth by ethanol precipitation, first and second Sephadex G-200 column chromatography with overall yield of 12%. The molecular weight of the enzyme was estimated to be 53,000 dalton by SDS-PAGE. The optimum pH and temperature was 8.5 and
토양에서 분리한 bilirubin oxidase를 생산하는 Penicillium sp. LAM 91-89의 배양액으로부터 효소를 정제하여 여러가지 성질을 조사하였다. LAM 91-89 billirubin oxidase는