Studies on chitobiase from Aeromonas salmonicida YA7-625

Aeromonas salmonicida YA7-625가 생산하는 Chitobiase의 순수분리와 특성

Chitobiase from Aeromonas salmonicida YA7-625 was purified from culture broth through ammonium sulfate precipitation, chitin affinity adsorption, hydroxylapatite column chromatography and gel filtration, with 47.2% yield and 31.5 fold purity. The molecular weight of purified chitobiase was 15,000 daltons, and the chitobiase showes maximum activity at the condition of at $40^{\circ}C$ and pH 6.0. The effects of various metal ions and inhibitors show thatcystein, glutamic acid, serine, tryptophan, and tyrosine residues seem to be concerned in chitobiase activity.

키틴 분해 효소활성이 높은 균주로 분리동정한 Aeromonas salmonicida YA7-625의 chitobiase를 ammonium sulfate침전, affinity adsorption, DEAE-cellulose chromatography, hydroxylapatite chromatography, gel filtration 과정을 통해 수율 47.2% 및 정제도 31.5로 분리, 정제 하였다. 정제된 chitobiase는 pH6.0, $40^{\circ}C$에서 최대의 활성을 나타내었고 분자량은 15,000 daltons로 추정되었다. 금속이온과 저해제들의 효과를 검토결과 효소의 활성에는 cystein, glutamic acid, aspartic acid, serine, tryptophan 및 tyrposine 잔기 등이 관여하는 것으로 유추되었다.