Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparison of Cultured Soymilk by Bifidobacterium and Various Human Intestinal Bacteria

Bifidobacterium과 기타 장내 세균에 의한 두유 배양 비교

  • Lee, Se-Kyung (Department of Food Science and Nutrition, Hallym University) ;
  • Son, Heon-Soo (Research Institute of Jung Food Company) ;
  • Ji, Geun-Eog (Department of Food Science and Nutrition, Hallym University)
  • 이세경 (한림대학교 식품영양학과) ;
  • 손헌수 (주식회사 정식품 기술연구소) ;
  • 지근억 (한림대학교 식품영양학과)
  • Published : 1993.12.01
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Abstract

Soymilk was cultured by various human large intestinal bacteria and lactic acid bacteria; Bifidobacterium longum, Lactobacillus acidophilus, Baeteroides fragilis, Eubacterium limosum, Clostridium perfringens and Escherichia coli. Among them, only B. longum utilized raffinose and stachyose actively which are major oligosaccharides present in soymilk by producing active ${\alpha}-galactosidase$ and produced greatest acid. Number of colony forming unit of B. longum reached $1.5{\times}10^{8}$ after 16 hr culture in soymilk. Also Bifidobacterium longum produced the highest level of ${\alpha}-galactosidase,\;{\beta}-galactosidase\;and\;{\alpha}-galactosidase$, in soymilk during culture.

Bifidobacterium과 기타 장내 세균을 두유에 배양하여 배양특성을 살펴보았다. 균주들은 다음과 같이 Bifidobacterium longum, Lactobacillus acidophilus, Bacteroides fragilis, Eubacterium limosum, Clostridium perfringens, E. coli.를 사용하였다. 이들중 B. longum만이 높은 활성의 ${\alpha}-galactosidase$를 생산해 두유의 주요 당인 raffinose와 stachyose를 이용하였고 가장 높은 산 생성을 보여주었다. 16시간 배양후에 B. longum은 $1.5{\times}10^{8}\;CFU$에 이르렀다. 또한 B. longum은 ${\alpha}-galactosidase$이외에 ${\alpha}-galactosidase$와 ${\beta}-galactosidase$의 활성도 다른 균주에 비하여 가장 높았다.

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