Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Determination of Isoflavonoid Glucosides in Rhizomes of Belamcanda chinensis by High Performance Liquid Chromatography

  • Cui, Jiong-Mo (Department of Pharmacognosy, Yanbian Medical College) ;
  • Chung, Ha-Sook (Natural Products Research Institute, Seoul National University) ;
  • Woo, Won-Sick (Natural Products Research Institute, Seoul National University)
  • Published : 1993.12.30
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Abstract

A new method for separation and quantitative determination of isoflavone glucosides in rhizomes of Belamcanda chinensis (Iridaceae) by high performance liquid chromatography was elaborated. A reverse-phase system with a Spheri-5 RP-18 column using MeOH:HOAc:$H_2O$(24 : 5 : 71) as a mobile phase was developed. The isoflavonoids were detected at 268 nm and the analysis was successfully carried out within 15 min.

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