The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Pectolytic Enzymes and Change of Pectic Substances from Apple Fruits Infected with Botryosphaeria dothidea

사과 겹무늬썩음병균(Botryosphaeria dothidea)에 의해 부패된 사과 과실에서 Pectin질 분해효소의 생산과 Pectin질의 변화

  • Park, Seok-Hee (Department of Holticulture, College of Agriculture and Animal Science Yeungnam University) ;
  • Lee, Chang-Un (Department of Holticulture, College of Agriculture and Animal Science Yeungnam University)
  • 박석희 (영남대학교 농축산대학 원예학과) ;
  • 이창은 (영남대학교 농축산대학 원예학과)
  • Published : 1993.06.30
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Abstract

Pectolytic enzymes were extracted in apple fruits rotted by Botryosphaeria dothidea, and their activities and change of pectic substances were investigated. Exo-polygalacturonase(exo-PG), exo-polymethylgalacturonase(exe-PMG), polygalacturonate-trans-eliminase(PGTE) and pectin-methyl-trans-eliminase(PMTE) were produced by the pathogen. Activities of exo-PG and exe-PMG extracted from rotten apple fruits were high to 21.15 and 24.65 units/mg protein in specific activity at seven days after inoculation, respectively. Activities of PGTE and PMTE showed 5.60 and 7.90 units/mg protein, respectively, but they were lower than those of the exo-type enzymes. Water-soluble and versene-soluble pectins were 11.50 mg/100 mg-AIS and 7.31 mg/100 mg-AIS at 14 days after inoculation, namely, they were increased by 4.23 and 2.16 mg/100 mg-AIS over those of sound apples, respectively. Total soluble pectic substances of rotten apple were 72.4% of total pectic substances and it was higher by 24.8% than sound apple. Insoluble pectic substance was notably decreased from 15.32 to 7.16 mg/100 mg-AIS according to progress of decay while total pectic substances were not changed remarkably.

사과 겹무늬썩음병균 Botryosphaeria dothidea에 의해 부패된 사과과실에서 pectin질 분해효소를 추출하여 그들의 활성과 pectin 성분의 변화를 조사하였다. 본 병원균은 exo-polygalacturonase(exo-PG), exo-polymethylgalacturonase(exo-PMG), polygalacturonate-trans -e1iminase(PGTE)와 pectinmethyl-trans-eliminase(PMTE)를 생산하였다. 부패된 사과 과육에서 exo-PG와 exo-PMG는 접종 후 7일째 specific activity가 각각 21.15 및 24.65 units/mg protein으로 높게 나타났다. PGTE와 PMTE의 활성은 7일째 각각 5.60과 7.90 uints/mg protein으로 나타났으나 exo-type의 효소보다는 그 활성이 낮았다. 수용성 pectin은 부패가 진행됨에 따라 14일째에 11.50 mg/100 mg-AIS이었고, versene-soluble pectin 역시 7.31 mg/100 mg-AIS로 나타나 건전과와 비교하여 각각 4.23 및 2.16 mg/100 mg-AIS 증가하였다. 부패과의 총 가용성 펙틴 함량은 총 pectin의 72.4%로서 건전과와 비교하여 24.8% 더 높았다. 불용성 pectin 함량은 부패가 진행됨에 따라 15.32 mg/100 mg-AIS에서 7.16 mg/100 mg-AIS로 현저하게 감소하였으며, 총 pectin 함량은 큰 변화가 없었다.

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