Clinical and Experimental Reproductive Medicine
- 제19권1호
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- Pages.57-64
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- 1992
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
TEST-Yolk Buffer에 의한 인간 정자의 수정능 증진효과에 관한 연구
Improved Fertilization Rate in Human In vitro Fertilization with the Use of a TEST-Yolk Buffer
- 방명걸 (서울대학교 의과대학 산부인과학교실) ;
- 김기철 (서울대학교 의과대학 산부인과학교실) ;
- 신창재 (서울대학교 의과대학 산부인과학교실) ;
- 문신용 (서울대학교 의과대학 산부인과학교실) ;
- 이진용 (서울대학교 의과대학 산부인과학교실) ;
- 장윤석 (서울대학교 의과대학 산부인과학교실)
- Pang, Myung-Geol (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Kim, Ki-Chul (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Shin, Chang-Jae (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Moon, Shin-Yong (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Lee, Jin-Yong (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Chang, Yoon-Seok (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University)
- 발행 : 1992.06.30
초록
The present study was undertaken to clarify the role of TEST-Yolk Buffer(TYB) as a factor for the improvement of human sperm fertility potential. We examined the effects of low temperature capacitation using TYB on sperm motility (%), motility pattern, normal morphology, true acrosome reaction, sperm penetration assay and human in vitro fertilization. Comparing the TYB method and swim-up method, the sperm motility(%) of selected sperm was not significantly different, but statistically significant differences were found in curvilinear velocity, linearity, lateral head displacement, normal morphology(%) and true acrosome reaction(%)(p<0.05). Results obtained from the sperm penetration assay demonstrated that the penetration index and penetration rate were increased significantly(p<0.05) when the spermatozoa were incubated in TYB, as compared with swim-up method. And fertilization of intact human oocytes was more succesful when spermatozoa were pretreated with TYB at
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